Details

Autor(en) / Beteiligte

• del Barrio, Melisa
• Guendon, Chloé
• Kpebe, Arlette
• Baffert, Carole
• Fourmond, Vincent
• Brugna, Myriam
• Léger, Christophe

Titel

Valine-to-Cysteine Mutation Further Increases the Oxygen Tolerance of Escherichia coli NiFe Hydrogenase Hyd‑1

Ist Teil von

• ACS catalysis, 2019-05, Vol.9 (5), p.4084-4088

Ort / Verlag

American Chemical Society

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Some NiFe hydrogenases are particularly resistant to O2 as a result of either the natural presence of a particular FeS cluster or the artificial replacement of a conserved valine residue near the Ni site. We show that the two protective effects can be combined in a single enzyme by constructing and characterizing the V78C variant of the naturally O2-tolerant Escherichia coli NiFe hydrogenase Hyd-1. We elucidated the effect of the mutation by comparing the kinetics of inhibition by CO and O2 of a number of wild-type forms and valine-to-cysteine variants of NiFe hydrogenases.