Details

Autor(en) / Beteiligte

• Assossou, Olga
• Besson, Françoise
• Rouault, Jean-Pierre
• Persat, Florence
• Brisson, Christine
• Duret, Laurent
• Ferrandiz, Josette
• Mayençon, Martine
• Peyron, François
• Picot, Stephane

Titel

Subcellular localization of 14-3-3 proteins in Toxoplasma gondii tachyzoites and evidence for a lipid raft-associated form

Ist Teil von

• FEMS microbiology letters, 2003-07, Vol.224 (2), p.161-168

Ort / Verlag

Oxford, UK: Elsevier B.V

Erscheinungsjahr

2003

Link zum Volltext

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• A polyclonal antibody was raised against a Toxoplasma gondii 14-3-3–gluthatione S-transferase fusion protein obtained by cloning a 14-3-3 cDNA sequence determined from the T. gondii database. This antibody specifically recognized T. gondii 14-3-3 without any cross-reaction with mammalian proteins. Immunofluorescence microscopy studies of the tachyzoites or the T. gondii-infected cells suggested cytosolic and membranous localizations of 14-3-3 protein. Different subcellular fractions were prepared for electrophoresis analysis and immunodetection. 14-3-3 proteins were found in the cytosol, the membrane fraction and Triton X-100-resistant membranes. Two 14-3-3 isoforms were detected. The major one was mainly cytoplasmic and to a lesser extent membrane-associated, whereas the minor isoform was associated with the detergent-resistant lipid rafts.