Autor(en)
Mihovilovic, Anita Bosnjak; Jercic, Ivanka Habus; Prebeg, Tatjana; Tomaz, Ivana; Pavicic, Antonio; and, Marijana Baric; Keresa, Snjezana
Titel
Light Source and Cytokinin Type Affect Multiplication Rate, Chlorophyll Content and Stomata Formation of Amelanchier alnifolia Shoots in Vitro/Izvor osvjetljenja i tip citokinina utjecu na stopu multiplikacije, sadrzaj klorofila i formiranje puci kod izdanaka Amelanchier alnifolia u in vitro uvjetima
Teil von
  • Journal of Central European agriculture, 2020-12-01, Vol.21 (4), p.826
Ort / Verlag
Sveuciliste U Zagrebu
Links zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen
Amelanchier alnifolia, a deciduous shrub or small tree with edible berry-like fruit, is gaining importance as a commercial fruit crop. The application of micropropagation could complement conventional propagation methods of A. alnifolia. The present study aimed to investigate how two light treatments-a mix of red and blue LED lights with dominant wavelengths of 457 and 658 nm (RB LED) and conventional fluorescent lamps emitting light at broad wavelengths of 400-700 nm (FL)-in combination with three cytokinins (CK) belonging to either substituted adenines (6-benzylaminopurine, BAP; meta-Topolin, mT) or to phenylurea cytokinins (thidiazurone, TDZ) affected the multiplication rate, chlorophyll content, and stomata formation in in vitro culture of A. alnifolia axillary shoots. The two light sources proved equally effective in producing axillary shoots, but FL favorably influenced the elongation and chlorophyll content. On the other hand, RB LED lights triggered a significantly higher stomata number of A. alnifolia plantlets in vitro compared with those developed under FL. Presented results reveal a negative ratio between the stomata number and chlorophyll content in response to different cytokinins: TDZ induced the highest number of stomata while BAP significantly increased the chlorophyll content. As the largest number of axillary shoots per explant was achieved on the medium with addition of TDZ, it could be beneficial to use TDZ for multiplication, whereas the use of BAP could be advantageous in the last passage of multiplication before rooting. Keywords: LED light, fluorescent light, cytokinin, BAP, mT, TDZ, in vitro propagation, chlorophyll content, stomata formation Amelanchier alnifolia je listopadni grm ili nize stablo s jestivim bobicastim plodovima radi kojih se sve vise komercijalno uzgaja. Primjena mikrorazmnozavanja mogla bi nadopuniti uobicajene metode razmnozavanja A. alnifolia. Ovo istrazivanje imalo je za cilj istraziti utjecaj dva tretmana svjetla - mjesavine crvenih i plavih LED svjetiljki s dominantnim valnim duljinama od 457 i 658 nm (RB LED) i konvencionalnih fluorescentnih lampi koje emitiraju svjetlost u sirokom rasponu valnih duljina od 400 do 700 nm (FL) - u kombinaciji s tri citokinina koji pripadaju bilo supstituiranim adeninima (6-benzilaminopurin, BAP i meta-topolin, mT) ili fenilurea citokininima (tidiazuron, TDZ) na stopu umnazanja, sadrzaj klorofila i formiranje puci u in vitro kulturi aksilarnih izdanaka A. alnifolia. Dva izvora svjetlosti pokazala su se jednako ucinkovita u stvaranju aksilarnih izdanaka, ali FL su povoljno utjecale na izduzivanje izdanaka i sadrzaj klorofila. S druge strane, pod RB LED svjetlima formirao se znacajno veci broj puci kod A. alnifolia izdanaka in vitro u usporedbi s onima razvijenim pod FL. Ovdje prikazani rezultati otkrivaju negativan omjer izmedu broja puci i sadrzaja klorofila pod utjecajem razlicitih citokinina: TDZ je inducirao stvaranje najveceg broja pud, dok je BAP znacajno povecao sadrzaj klorofila. S obzirom da je najveci broj aksilarnih izdanaka po eksplantatu formiran na mediju s dodatkom TDZ-a, TDZ je pogodan u fazi multiplikacije, dok bi se pozitivan utjecaj BAP-a mogao iskoristiti u posljednjoj supkultivaciji prije ukorjenjivanja. Kljucne rijeci: LED svjetlo, fluorescentno svjetlo, citokinin, BAP, mT, TDZ, in vitro raznmozavanje, sadrzaj klorofila, formiranje puci
Format
Sprache(n)
Englisch
Identifikator(en)
ISSN: 1332-9049
ISSN: 1332-9049
DOI: 10.5513/JCEA01/21.4.2909
Links zum Inhalt
Schlagwörter
Chlorophyll, Cytokinins, Fruit, Light-emitting diodes, Usage
Systemstelle
Signatur

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