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Green synthesis of silver nanoparticles from medicinal plants and evaluation of their antiviral potential against chikungunya virus
Ist Teil von
Applied microbiology and biotechnology, 2019-01, Vol.103 (2), p.881-891
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2019
Link zum Volltext
Quelle
SpringerLink
Beschreibungen/Notizen
The exploration of nanoscale materials for their therapeutic potential against emerging and re-emerging infections has been increased in recent years. Silver nanoparticles (AgNPs) are known to possess antimicrobial activities against different pathogens including viruses and provide an excellent opportunity to develop new antivirals. The present study focused on biological synthesis of AgNPs from
Andrographis paniculata
,
Phyllanthus niruri
, and
Tinospora cordifolia
and evaluation of their antiviral properties against chikungunya virus. Synthesized plants AgNPs were characterized to assess their formation, morphology, and stability. The cytotoxicity assays in Vero cells revealed that
A. paniculata
AgNPs were most cytotoxic with maximum non-toxic dose (MNTD) value of 31.25 μg/mL followed by
P. niruri
(MNTD, 125 μg/mL) and
T. cordifolia
AgNPs (MNTD, 250 μg/mL). In vitro antiviral assay of AgNPs based on degree of inhibition of cytopathic effect (CPE) showed that
A. paniculata
AgNPs were most effective, followed by
T. cordifolia
and
P. niruri
AgNPs
.
The results of antiviral assay were confirmed by cell viability test using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) dye, which revealed that
A. paniculata
AgNPs inhibited the virus to a maximum extent. The cell viability of CHIKV-infected cells significantly increased from 25.69% to 80.76 and 66.8%, when treated with
A. paniculata
AgNPs at MNTD and ½MNTD, respectively. These results indicated that use of plants AgNPs as antiviral agents is feasible and could provide alternative treatment options against viral diseases which have no specific antiviral or vaccines available yet.