Details

Autor(en) / Beteiligte

• Kim, S.S. (Myongji University, Yongin, Republic of Korea)
• Suh, J.W. (Myongji University, Yongin, Republic of Korea)
• Kim, J.Y. (Jeju Biodiversity Research Institute (JBRI), Jeju, Republic of Korea)
• Hyun, C.G. (Jeju Biodiversity Research Institute (JBRI), Jeju, Republic of Korea), E-mail: cghyun@jejuhidi.or.kr

Titel

Molecular Cloning, Sequencing, and Biochemical Characterization of Streptomyces rimosus TDP-glucose 4,6-dehydratase

Ist Teil von

• Journal of applied biological chemistry, 2007-09, Vol.50 (3)

Erscheinungsjahr

2007

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• TDP-(Thymidine diphosphate)-glucose 4,6-dehydratase (EC 4.2.1.46), which converts TDP-glucose to TDP-4-keto-6-deoxyglucose, is a common essential enzyme in the biosynthesis of all types of 6-deoxyhexose. Genes that encode TDP-glucose 4,6-dehydratase are found within gene clusters that contain biosynthetic genes for natural products containing 6-deoxyhexose moieties. The genes that are required for the bioxynthesis of secondary metabolites, lipopolysaccharide, and O-antigen are also usually clustered in the genome in competent organisms [Kim et al., 2000]. In our previous work, we therefore designed oligonucleotide primers to amplify DNA fragments of the genes for actinomycetes TDP-glucose synthase, which could then be used to efficiently amplify and isolate the genes for sugar moiety biosynthesis in actinomycetes [Hyun et al., 2000].