Details

Autor(en) / Beteiligte

• Qin, Xiaojie
• Liu, Yanhong
• Shi, Xianming

Titel

Resistance-Nodulation-Cell Division (RND) Transporter AcrD Confers Resistance to Egg White in Salmonella enterica Serovar Enteritidis

Ist Teil von

• Foods, 2021-12, Vol.11 (1), p.90

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The excellent survival ability of serovar Enteritidis ( . Enteritidis) in egg white leads to outbreaks of salmonellosis frequently associated with eggs and egg products. Our previous proteomic study showed that the expression of multidrug efflux RND transporter AcrD in . Enteritidis was significantly up-regulated (4.06-fold) in response to an egg white environment. In this study, the potential role of AcrD in the resistance of . Enteritidis to egg white was explored by gene deletion, survival ability test, morphological observation, Caco-2 cell adhesion and invasion. It was found that deletion of had no apparent effect on the growth of . Enteritidis in Luria-Bertani (LB) broth but resulted in a significant ( < 0.05) decrease in resistance of . Enteritidis to egg white and a small number of cell lysis. Compared to the wild type, a 2-log population reduction was noticed in the mutant with different initial concentrations after incubation with egg white for 3 days. Furthermore, no significant difference ( > 0.05) in the adhesion and invasion was found between the wild type and mutant in LB broth and egg white, but the invasion ability of the mutant in egg white was significantly ( < 0.05) lower than that in LB broth. This indicates that is involved in virulence in . Taken together, these results reveal the importance of AcrD on the resistance of . Enteritidis to egg white.