Autor(en)
QIAN Jun; CAO Yi; LI Lingchang; YU Jialin; HUO Jiege
Titel
Mechanism of lncRNA JPX targeting miR-378 in tanshinone ⅡA affecting proliferation, cloning and apoptosis in colon cancer SW620 cells
Teil von
  • Di 3 jun yi da xue xue bao, 2021-05-01, Vol.43 (9), p.837-844
Ort / Verlag
Editorial Office of Journal of Third Military Medical University
Links zum Volltext
Quelle
DOAJ Directory of Open Access Journals
Beschreibungen
Objective To study the mechanism of lncRNA JPX targeting miR-378 in the proliferation, cloning and apoptosis of colon cancer SW620 cells induced by tanshinone ⅡA. Methods Colon cancer SW620 cells were divided into control group, tanshinone ⅡA treatment group, vector group(transfected with negative control vector), JPX group(transfected with pcDNA-JPX), tanshinone ⅡA+vector group(transfected with negative control vector followed by tanshinoneⅡA treatment), tanshinoneⅡA+JPX group(transfected with pcDNA-JPX followed by tanshinoneⅡA treatment)), tanshinoneⅡA+JPX+miR-NC group(cotransfected with pcDNA-JPX and mimics control, followed by tanshinoneⅡA treatment), tanshinoneⅡA+ JPX+miR-378 group(cotransfected with pcDNA-JPX and miR-378 mimics, then treated with tanshinoneⅡA), tanshinoneⅡA+anti-NC group(transfected with inhibitor control followed by tanshinoneⅡA treatment), tanshinoneⅡA+anti-miR-378 group(transfected with miR-378 inhibitor followed by tanshinoneⅡA treatment). CCK-8 assay was used to detect cell prolife
Format
Sprache(n)
Chinesisch
Identifikator(en)
ISSN: 1000-5404
DOI: 10.16016/j.1000-5404.202011068
Links zum Inhalt
Schlagwörter
apoptosis, colon cancer, jpx, mir-378, tanshinone ⅱa
Systemstelle
Signatur
Die Universität der Informationsgesellschaft