eLife, 2018-10, Vol.7
- Fischer, Juliane
- Müller, Sebastian Y
- Netzker, Tina
- Jäger, Nils
- Gacek-Matthews, Agnieszka
- Scherlach, Kirstin
- Stroe, Maria C
- García-Altares, María
- Pezzini, Francesco
- Schoeler, Hanno
- Reichelt, Michael
- Gershenzon, Jonathan
- Krespach, Mario Kc
- Shelest, Ekaterina
- Schroeckh, Volker
- Valiante, Vito
- Heinzel, Thorsten
- Hertweck, Christian
- Strauss, Joseph
- Brakhage, Axel A
2018
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- Autor(en) / Beteiligte
- Fischer, Juliane
- Müller, Sebastian Y
- Netzker, Tina
- Jäger, Nils
- Gacek-Matthews, Agnieszka
- Scherlach, Kirstin
- Stroe, Maria C
- García-Altares, María
- Pezzini, Francesco
- Schoeler, Hanno
- Reichelt, Michael
- Gershenzon, Jonathan
- Krespach, Mario Kc
- Shelest, Ekaterina
- Schroeckh, Volker
- Valiante, Vito
- Heinzel, Thorsten
- Hertweck, Christian
- Strauss, Joseph
- Brakhage, Axel A
- Titel
- Chromatin mapping identifies BasR, a key regulator of bacteria-triggered production of fungal secondary metabolites
- Ist Teil von
- eLife, 2018-10, Vol.7
- Ort / Verlag
- England: eLife Science Publications, Ltd
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The eukaryotic epigenetic machinery can be modified by bacteria to reprogram the response of eukaryotes during their interaction with microorganisms. We discovered that the bacterium triggered increased chromatin acetylation and thus activation of the silent secondary metabolism gene cluster in the fungus . Using this model, we aim understanding mechanisms of microbial communication based on bacteria-triggered chromatin modification. Using genome-wide ChIP-seq analysis of acetylated histone H3, we uncovered the unique chromatin landscape in upon co-cultivation with and relate changes in the acetylation to that in the fungal transcriptome. Differentially acetylated histones were detected in genes involved in secondary metabolism, in amino acid and nitrogen metabolism, in signaling, and encoding transcription factors. Further molecular analyses identified the Myb-like transcription factor BasR as the regulatory node for transduction of the bacterial signal in the fungus and show its function is conserved in other species.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2050-084XeISSN: 2050-084XDOI: 10.7554/elife.40969Titel-ID: cdi_doaj_primary_oai_doaj_org_article_cafdcfa42f084b8b805c8be272eef709
- Format
- –
- Schlagworte
- Acetylation, Amino acids, Analysis, Aspergillus nidulans, Aspergillus nidulans - genetics, Aspergillus nidulans - metabolism, Bacteria, Biochemistry, Biology, Chromatin, Chromatin - metabolism, Chromosomes, DNA binding proteins, Epigenetic inheritance, Epigenetics, Fungal Proteins - genetics, Fungal Proteins - metabolism, Fungi, Gene expression, Gene Expression Regulation, Fungal, Gene mapping, Gene Ontology, Genes, Genome, Fungal, genome-wide dual ChIP-seq, Genomes, Genomics, Histidine - metabolism, Histone H3, histone modification, Histones, Histones - metabolism, Infections, Lysine - metabolism, Maximum likelihood method, Metabolites, microbial interaction, Microbiology and Infectious Disease, Microorganisms, Mitochondria - metabolism, Multigene Family, Natural products, Nitrogen - metabolism, Phylogenetics, Phylogeny, Physiological aspects, Plant metabolites, Schiller, Johann Christoph Friedrich von (1759-1805), Secondary Metabolism, Secondary metabolites, Signal Transduction, Streptomyces - metabolism, Transcription factors, Transcription Factors - metabolism, Wildlife conservation