Details

Autor(en) / Beteiligte

• Rupaimoole, Rajesha
• Lee, Jaehyuk
• Haemmerle, Monika
• Ling, Hui
• Previs, Rebecca A.
• Pradeep, Sunila
• Wu, Sherry Y.
• Ivan, Cristina
• Ferracin, Manuela
• Dennison, Jennifer B.
• Millward, Niki M. Zacharias
• Nagaraja, Archana S.
• Gharpure, Kshipra M.
• McGuire, Michael
• Sam, Nidhin
• Armaiz-Pena, Guillermo N.
• Sadaoui, Nouara C.
• Rodriguez-Aguayo, Cristian
• Calin, George A.
• Drapkin, Ronny I.
• Kovacs, Jeffery
• Mills, Gordon B.
• Zhang, Wei
• Lopez-Berestein, Gabriel
• Bhattacharya, Pratip K.
• Sood, Anil K.

Titel

Long Noncoding RNA Ceruloplasmin Promotes Cancer Growth by Altering Glycolysis

Ist Teil von

• Cell reports (Cambridge), 2015-12, Vol.13 (11), p.2395-2402

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• Long noncoding RNAs (lncRNAs) significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP) in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer. [Display omitted] •Long noncoding RNA NRCP is upregulated in ovarian cancer•NRCP promotes cancer cell progression by promoting glycolysis•Silencing NRCP results in apoptosis and decreased tumor progression The lncRNA NRCP is upregulated in ovarian cancer. Rupaimoole et al. show that NRCP is involved in cancer cell glycolysis and promotes tumor growth and proliferation. They find that silencing NRCP using DOPC-siRNAs in vivo results in robust anti-tumor effects.