Details

Autor(en) / Beteiligte

• Youssef, Fadia S
• Altyar, Ahmed E
• Omar, Abdelsattar M
• Ashour, Mohamed L

Titel

Phytoconstituents, In Vitro Anti-Infective Activity of Buddleja indica Lam., and In Silico Evaluation of its SARS-CoV-2 Inhibitory Potential

Ist Teil von

• Frontiers in pharmacology, 2021-04, Vol.12, p.619373-619373

Ort / Verlag

Switzerland: Frontiers Media S.A

Erscheinungsjahr

2021

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Phytochemical investigation of Lam. leaves methanol extract (BIT) resulted in the isolation of six known compounds for the first time from the plant, namely, -hydroxybenzoic acid 1), caffeic acid 2), quercetin 3-O- D glucoside-7-O-α-L-rhamnoside 3), kaempferol 3-O- D glucoside-7-O-α-L-rhamnoside 4), quercetin 7-O- D glucoside 5) and kaempferol 6). BIT extract showed potent antibacterial activity with MIC values ranging between 0.48 and 1.95 μg/ml with was the most susceptible to the BIT effect. It showed a notable antimycobacterial and anti- activity with MIC values of 100 and 80 μg/ml, respectively. Vesicular stomatitis virus (VSV) was more sensitive to the antiviral activity of BIT comparable to herpes simplex virus type 1 (HSV-1), showing 48.38 and 41.85% inhibition of the viral replication at a dose of 50 μg/ml for VSV and HSV-1, respectively. molecular docking of the isolated compounds revealed that caffeic acid 2) showed the highest fitting within the active sites of DNA-gyrase, topoisomerase IV, and SARS-CoV-2 M . Quercetin 7-O- D glucoside 5) revealed the best fitting in dihydrofolate reductase active site with ∆ G value equals -36.53 Kcal/mol. Kaempferol 6) exhibited the highest fitting towards -lactamase, SARS-CoV-2PL , and SARS-CoV-2 3CL active sites. Thus, Lam. can be considered as a future source of cheap, substantially safe, and credible antibacterial, antifungal, and antiviral candidate of natural origin that could effectively participate in solving the problem of COVID-19 pandemic. These findings provide a scientific consolidation for the ethnomedicinal uses of Lam. as a topical antiseptic.