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Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance
Ist Teil von
International journal of molecular sciences, 2018-02, Vol.19 (2), p.512
Ort / Verlag
Switzerland: MDPI AG
Erscheinungsjahr
2018
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
The transcription factor
has been implicated in cartilage formation and testis determination in mammals. Here, two duplicates of
were found in Japanese flounder (
) named
and
, respectively. Phylogenetic and gene structure analyses revealed that
and
were homologous to that of teleosts and tetrapods. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that both
and
expressed higher in testis than in ovary of adult tissues. The in situ hybridization (ISH) analysis of gonads showed that
and
mRNA were both detected in oocytes, Sertoli cells and spermatocytes. During sex differentiation, the expression of
exhibited obvious sexual dimorphic expression from 60 days after hatch (dah) with higher expression in male preferred individuals than female preferred individuals and increased gradually from 30 to 100 dah. A similar pattern was detected in
expression. After injection of androgen (17α-methyltestosterone) of different concentrations, the expression level of
increased significantly, whereas
did not change obviously. These results indicated that the two
genes of Japanese flounder had converse functions in sex differentiation, whereas their differences in 17α-methyltestosterone administration were obvious and worthwhile for exploring evolutionary and adaptive significance. This study provided a foundation for further exploration of the roles of
genes during the sex determination and differentiation, spermatogenesis and gonadal function maintenance of Japanese flounder.