Details

Autor(en) / Beteiligte

• Gu, Yuchen
• Liang, Ying
• Bai, Jie
• Wu, Wei
• Lin, Qinlu
• Wu, Jianping

Titel

Spent hen-derived ACE inhibitory peptide IWHHT shows antioxidative and anti-inflammatory activities in endothelial cells

Ist Teil von

• Journal of functional foods, 2019-02, Vol.53, p.85-92

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

2019

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •IWHHT is a spent hen derived ACE inhibitory peptide.•IWHHT can be further degraded into IW and IWH.•IWHHT and its digest peptide IWH showed anti-inflammatory activity while IWH and IW showed antioxidant activity. The pathophysiology of hypertension involves the interplay among renin-angiotensin system (RAS), oxidative stress and inflammation. Previous studies focused mainly on ACE inhibitory activity; the goals of this study were to characterize ACE inhibitory peptides from chicken and to investigate their potential antioxidative and anti-inflammatory activities. Spent hen muscle protein hydrolysate digested by thermolysin showed an IC50 value of 39.6 ± 2.3 µg/mL. Following HPLC fractionation and LC-MS/MS analysis, a potent ACE inhibitory peptide IWHHT (IC50 = 9.93 ± 0.65 µM) was identified. IWHHT was not resistant to simulated gastrointestinal digestion and produced a mixture of IW and IWH. IWHHT and IWH significantly reduced the expression of vascular cell adhesion molecule-1 (VCAM-1) in tumor necrosis factor-alpha (TNF-α)-induced inflammation. IWHHT, IWH and IW also significantly reduced superoxide level in basal oxidation test. The multi-activities of IWHHT and its associated digestion products may enhance beneficial actions against hypertension.