Molecular cancer, 2020-01, Vol.19 (1), p.9-9, Article 9
2020
Details
- Autor(en) / Beteiligte
- Titel
- lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling
- Ist Teil von
- Molecular cancer, 2020-01, Vol.19 (1), p.9-9, Article 9
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2020
- Link zum Volltext
- Quelle
- SpringerNature Journals
- Beschreibungen/Notizen
- MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown. RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. We observed that lncRNA JPX was upregulated in lung cancer metastatic tissues and was closely correlated with tumor size and an advanced stage. Functionally, JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in vivo. Additionally, JPX upregulated Twist1 by competitively sponging miR-33a-5p and subsequently induced EMT and lung cancer cell invasion. Interestingly, JPX and Twist1 were coordinately upregulated in lung cancer tissues and cells. Mechanically, the JPX/miR-33a-5p/Twist1 axis participated in EMT progression by activating Wnt/β-catenin signaling. These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1476-4598eISSN: 1476-4598DOI: 10.1186/s12943-020-1133-9Titel-ID: cdi_doaj_primary_oai_doaj_org_article_3a08fa94335c4691853551964d2d1b07
- Format
- –
- Schlagworte
- Adenocarcinoma of Lung - genetics, Adenocarcinoma of Lung - metabolism, Adenocarcinoma of Lung - secondary, Animals, Apoptosis, beta Catenin - genetics, beta Catenin - metabolism, Binding sites, Biomarkers, Tumor, Cancer therapies, Carcinoma, Squamous Cell - genetics, Carcinoma, Squamous Cell - metabolism, Carcinoma, Squamous Cell - secondary, Cell culture, Cell growth, Cell lines, Cell Movement, Cell Proliferation, Epithelial-mesenchymal transition, Female, Flow cytometry, Gene expression, Gene Expression Regulation, Neoplastic, Humans, Laboratory animals, Long noncoding RNA, Lung cancer, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Male, Mesenchyme, Metastases, Metastasis, Mice, Mice, Inbred BALB C, Mice, Nude, MicroRNAs, MicroRNAs - genetics, Middle Aged, miRNA, Neoplasm Invasiveness, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Plasmids, Prognosis, Proteins, RNA, Long Noncoding - genetics, Survival Rate, Tissues, Tumor cell lines, Tumor Cells, Cultured, Tumorigenesis, Twist-Related Protein 1 - genetics, Twist-Related Protein 1 - metabolism, Twist1, Western blotting, Wnt protein, Wnt Proteins - genetics, Wnt Proteins - metabolism, Wnt/β-catenin signaling, Xenograft Model Antitumor Assays, Xenografts, β-Catenin