Details
- Autor(en) / Beteiligte
- Titel
- Beneficial Effect of Jojoba Seed Extracts on Hyperglycemia-Induced Oxidative Stress in RINm5f Beta Cells
- Ist Teil von
- Nutrients, 2018-03, Vol.10 (3), p.384
- Ort / Verlag
- Switzerland: MDPI AG
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Hyperglycemia occurs during diabetes and insulin resistance. It causes oxidative stress by increasing reactive oxygen species (ROS) levels, leading to cellular damage. Polyphenols play a central role in defense against oxidative stress. In our study, we investigated the antioxidant properties of simmondsin, a pure molecule present in jojoba seeds, and of the aqueous extract of jojoba seeds on fructose-induced oxidative stress in RINm5f beta cells. The exposure of RINm5f beta cells to fructose triggered the loss of cell viability (-48%, < 0.001) and disruption of insulin secretion ( < 0.001) associated with of reactive oxygen species (ROS) production and a modulation of pro-oxidant and antioxidant signaling pathway. Cell pre-treatments with extracts considerably increased cell viability (+86% < 0.001) for simmondsin and +74% ( < 0.001) for aqueous extract and insulin secretion. The extracts also markedly decreased ROS (-69% ( < 0.001) for simmondsin and -59% ( < 0.001) for aqueous extract) and caspase-3 activation and improved antioxidant defense, inhibiting p22phox and increasing nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels (+70%, < 0.001) for aqueous extract. Simmondsin had no impact on Nrf2 levels. The richness and diversity of molecules present in jojoba seed extract makes jojoba a powerful agent to prevent the destruction of RINm5f beta cells induced by hyperglycemia.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2072-6643eISSN: 2072-6643DOI: 10.3390/nu10030384Titel-ID: cdi_doaj_primary_oai_doaj_org_article_396138b071db4cb997cc8a854d5420e7
- Format
- –
- Schlagworte
- Acetonitriles - isolation & purification, Acetonitriles - pharmacology, Animals, Antioxidants, Antioxidants - isolation & purification, Antioxidants - pharmacology, Beta cells, Caspase, Caspase 3 - metabolism, Caspase-3, Cell Line, Tumor, Cell Survival - drug effects, Cyclohexanes - isolation & purification, Cyclohexanes - pharmacology, Diabetes mellitus, Dose-Response Relationship, Drug, Fructose, Fructose - toxicity, Glucosides - isolation & purification, Glucosides - pharmacology, Hyperglycemia, Insulin, Insulin - metabolism, Insulin Secretion, Insulin-Secreting Cells - drug effects, Insulin-Secreting Cells - metabolism, Insulin-Secreting Cells - pathology, jojoba, Magnoliopsida - chemistry, NADPH Oxidases - metabolism, NF-E2-Related Factor 2 - metabolism, Nrf2, Oxidation resistance, Oxidative stress, Oxidative Stress - drug effects, p22phox, Phytotherapy, Plant Extracts - isolation & purification, Plant Extracts - pharmacology, Plants, Medicinal, Polyphenols, Rats, Reactive Oxygen Species - metabolism, RINm5f, Secretion, Seeds, Seeds - chemistry, Signal transduction, Signal Transduction - drug effects, Signaling, Simmondsin