Details

Autor(en) / Beteiligte

• Zhang, Hui
• Hao, Yuanyuan
• Wei, Cong
• Yao, Bing
• Liu, Shen
• Zhou, Hongru
• Huang, Dan
• Zhang, Chuanhai
• Wu, Yiling

Titel

Chinese medicine Jinlida granules improve high-fat-diet induced metabolic disorders via activation of brown adipose tissue in mice

Ist Teil von

• Biomedicine & pharmacotherapy, 2019-06, Vol.114, p.108781-108781, Article 108781

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• •JLD ameliorated HFD-induced obesity and fat mass gain in mice.•JLD regulated glucose and lipid metabolism in HFD-induced obese mice.•JLD improved HFD-induced hepatic steatosis and inflammation in mice.•JLD activated BAT thermogenesis by promoting mitochondrial biogenesis and fatty acid oxidation in HFD-induced obese mice. Activation of brown adipose tissue (BAT) thermogenesis could contribute to energy expenditure, which is critical for the treatment of obesity and type 2 diabetes mellitus (T2DM). In the present study, we aimed to systematically investigate whether traditional Chinese medication Jinlida (JLD) granules could improve metabolic disorders and activate BAT thermogenesis in C57BL/6 J mice fed with a high-fat diet (HFD). In the present study, JLD (3.8 g/kg) in 0.5% of carboxymethyl cellulose (CMC) solution was administrated daily by oral gavage to HFD-induced mice for 15 weeks. The body weight, biochemical analysis, histology analysis, intraperitoneal glucose and insulin tolerance (OGTT and ITT) tests were measured to explore metabolic disorders. Cold tolerance test, real-time PCR (qRT-PCR), immunohistochemistry, and western blot were performed to evaluate BAT function. As results, JLD treatment significantly ameliorated HFD-induced obesity and fat mass gain, maintained glucose and lipid homeostasis, and improved hepatic steatosis and inflammation. More importantly, we observed that JLD markedly activated BAT thermogenesis in HFD-induced obese mice. Moreover, our data confirmed that JLD promoted mitochondrial biogenesis and fatty acid oxidation metabolism in BAT. These data suggested that JLD could improve metabolic disorders in associated with activation of BAT thermogenesis via enhancement of mitochondrial biogenesis and fatty acid oxidation metabolism, thus providing a new pharmacological evidence for the clinical usage of JLD in T2DM treatment.