Details

Autor(en) / Beteiligte

• Zhang, Tian Xing
• Zhu, Feng
• Wang, Qing Ming
• Tang, Xin Hui
• Liu, Jia

Titel

The Investigation of the Interaction between Daidzin and Bovine Serum Albumin by Fluorescence Spectroscopy

Ist Teil von

• Applied Mechanics and Materials, 2014-10, Vol.664 (Mechatronics and Mechanical Engineering I), p.402-409

Ort / Verlag

Zurich: Trans Tech Publications Ltd

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We investigated the mutual interaction of daidzin with bovine serum albumin (BSA) by fluorescence spectroscopy. The results revealed that daidzin cause the fluorescence quenching of BSA through a static quenching procedure. The Stern-Volmer quenching constant (Ksv) were calculated at different temperature. The binding site (n), apparent binding constant (Ka) and corresponding thermodynamic parameters △Go, △Ho, △So were calculated and the van der Waals interaction, hydrogen bonds and hydrophobic interactions play an important role in stabilizing the complex. Besides, we also studied the effect of Cu2+, Ni2+, Mn2+ and Co2+ on the binding constants between daidzin and BSA, it is shows that the binding of BSA and daidzin is strengthened in the presence metal ions.