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Basal respiration is one of the key indicators of soil C mineralization. Temperature sensitivity (Q
10
) of basal respiration is important for predicting changes in C mineralization due to warming. A modified methodology of Q
10
determination is proposed. Soil samples were incubated at 25°C with periodic short-term (2 h) decline of temperature to 15°C and high-frequency measurements of CO
2
production rates. The temperature sensitivity is estimated as the average rate of CO
2
production at 25°C (before and after temperature decline) divided by the rate of CO
2
production at 15°C. With this method we demonstrated that glucose addition most strongly affects the Q
10
values at low temperature ranges (20–10°C), while temperature range affects Q
10
stronger than the glucose additions. The negative effect of soil moisture on Q
10
of basal respiration was demonstrated: the Q
10
values decreased with increasing soil moisture.