Details

Autor(en) / Beteiligte

• Lahoz, B
• Alabart, JL
• Folch, J
• Sánchez, P
• Echegoyen, E
• Cocero, MJ

Titel

Influence of the Fec X R Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU ‐Derived Oocytes

Ist Teil von

• Reproduction in domestic animals, 2013-10, Vol.48 (5), p.717-723

Erscheinungsjahr

2013

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Contents Ewes heterozygous for the F ec X R allele (R+) in the bone morphogenetic protein 15 ( BMP 15 ) gene display increased ovulation rate and prolificacy. Besides this phenotypic advantage, the influence of the F ec X R allele on follicle number and size, oocyte competence and in vitro production ( IVP ) remains undefined. With these aims, 8 R+ and 8 wild‐type (++) ewes were subjected to 2 laparoscopic ovum pick‐up ( LOPU ) trials (four sessions per trial; two with and two without FSH ) and subsequent IVP and fresh embryo transfer. All follicles >3 mm were punctured (n = 1673). Genotype did not significantly affect the number of punctured follicles per ewe and session (10.4 and 10.2 in R+ and ++ untreated ewes, 17.4 and 14.3 in R+ and ++ FSH ‐treated ewes, respectively), but follicular diameter of R+ ewes was significantly reduced compared with ++ ewes (−0.2 mm in untreated and −0.8 mm in FSH ‐treated ewes; p < 0.01). R+ ewes showed higher recovery rate and increased numbers of total and suitable cumulus–oocyte complexes for in vitro maturation (IVM). Similar rates of day 8 blastocysts were observed in R+ (36.1%, 147/407) and ++ (32.6%, 100/307) ewes, but the final output of day 8 blastocysts per ewe and session was higher in R+ ewes (+0.75; p < 0.005), without differences in survival rate at birth of the transferred embryos (40.4%, 21/52 vs 36.4%, 16/44, respectively). In conclusion, a higher number of oocytes proven to be competent for in vitro development and embryo survival after transfer are recovered from R+ ewes, despite the lower mean size of their follicles at puncture.