Details

Autor(en) / Beteiligte

• Wilkinson, J Erby
• Smith, Christina
• Suter, Maja
• Lewis, Robert M

Titel

Long-Term Cultivation of Canine Keratinocytes

Ist Teil von

• Journal of investigative dermatology, 1987-02, Vol.88 (2), p.202-206

Ort / Verlag

Danvers, MA: Elsevier Inc

Erscheinungsjahr

1987

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The growth characteristics and morphology of canine keratinocytes grown in vitro were studied. Keratinocytes from canine oral mucosa, ear skin, and ventral abdominal skin were grown in culture either as explants or as dispase/trypsin-derived suspensions in the absence of a feeder cell layer. Cholera toxin and epidermal growth factor were essential to the cells during multiple passages. Keratinocytes from all tissue sources, either as primary cultures or sub-cultivated for up to 10 passages, had growth characteristics and morphology similar to that reported in other species. The use of cultured canine keratinocytes should provide a suitable model for comparative in vitro studies of the pathogenesis of dermatologic diseases.