Details

Autor(en) / Beteiligte

• Schultz, Shannon L
• Birckbichler, Paul J

Titel

Cell Growth and Transglutaminase 2 Expression within the Human Erythroleukemia Cell Line (HEL)

Ist Teil von

• The FASEB journal, 2019-04, Vol.33 (S1), p.646.15-646.15

Ort / Verlag

The Federation of American Societies for Experimental Biology

Erscheinungsjahr

2019

Link zum Volltext

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• The objective of the study was to explore the biological responses of retinoic acid and sodium butyrate on human erythroleukemia cells (HEL). The process of apoptosis is exemplary in that the cytoskeleton collapses and the DNA is broken into fragments. The cellular components of the dead cell after undergoing apoptosis are able to be recycled. Furthermore, a cell that has experienced apoptosis does not disturb its neighbors. By learning the basics of human cell experimentation, further analysis was conducted of the HEL cell lines, such as an apoptotic assay and indirect immunofluorescence. Cells were treated with either buffer (control), retinoic acid (20 uM), sodium butyrate (100 uM) or a combination of retinoic acid (20 uM) and sodium butyrate (100 uM). Cell growth rates declined when the cells were treated with the chemicals; the greatest difference being in the combination culture of retinoic acid and sodium butyrate. The enzyme transglutaminase (TG2) is an enzyme found in large amounts within some cancer cells, and is known to have multiple functions. When the cells were treated with a monoclonal antibody to TG2, transglutaminase enzyme fluorescence in the control cultures were observed to be cytoplasmic. The retinoic acid‐treated cultures appeared to have a strong presence of the enzyme in the cytoplasm. The amount of TG2 appeared to increase in all the cultures with time with the greatest increase occurring in the combination cultures. After 144 hours a portion of the combination cultures had been converted to apoptotic bodies. Future research will attempt to localize the actual location of the transglutaminase when treated with both chemicals, and if it promotes or suppresses the viability of the cancer cells. Studies are underway to determine the amount of transglutaminase that is present in each of the samples. Support or Funding Information Funding provided by Department of Chemistry, Slippery Rock University This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal.