Non-edible Jojoba biodiesel blended with diesel was tested for use as a substitute fuel for diesel engines. The main objective of this study was to analyse the combustion and emission characteristics of Jojoba biodiesel blends (5%, 10% and 20%) and compare the results with that of standard diesel to identify suitable alternatives. Experiments were performed at 50% and 100% load for the speed range of 1200 rpm to 2400 rpm. The combustion parameters, namely cylinder pressure at various crank angles, heat release rate, ignition delay and the emission parameters, namely NOx, CO, CO2 and HC are analysed and discussed. The results indicated that Jojoba biodiesel can be used as an alternative for diesel fuel and 10% Jojoba biodiesel- diesel blend (i.e. JB10) behaves more like diesel than other blends, therefore, JB10 is considered as most suitable alternative to diesel. However, JB10 produces more NOx and CO2 emissions than diesel, which can be reduced by applying different combustion strategies in future.