Details

Autor(en) / Beteiligte

• Chauhan, Madhu
• Thota, Chandra S.
• Kondapaka, Sudhir
• Wimalawansa, Sunil
• Yallampalli, Chandra

Titel

Evidence for the existence of a new receptor for CGRP, which is not CRLR

Ist Teil von

• Peptides (New York, N.Y. : 1980), 2003, Vol.24 (1), p.65-71

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2003

Quelle

MEDLINE

Beschreibungen/Notizen

• Receptors for calcitonin gene-related peptide (CGRP), a neuropeptide known to be the most potent vasodilator, are abundantly expressed in cerebellum. A monoclonal antibody to cerebellar CGRP receptors specifically detects a 66 kDa protein from rat cerebellum and other rat and human tissues, but not from SK-N-MC cells which express calcitonin receptor-like receptor (CRLR), a recently described component of CGRP receptors. In contrast, mRNA expression for CRLR was abundant in SK-N-MC cells, but it was undetectable in rat cerebellum. Furthermore, the antibody could not detect any immunoreactive protein in HEK 293 cells transiently transfected with CRLR and receptor activity-modifying protein 1 (RAMP 1) indicating the possible existence of another CGRP receptor, which does not involve CRLR. Due to the absence of biochemical or structural data on the existence of a CGRP 2 receptor and the new data provided in this paper, we suggest to identify the two CGRP receptors as CGRP-A and CGRP-B.