Details

Autor(en) / Beteiligte

• Szarka, L.J.
• Cino, P.M.
• Ko, R.Y.

Titel

Penicillin fermentation using hydrocarbons

Ist Teil von

• Process biochemistry (1991), 1992-03, Vol.27 (2), p.77-82

Ort / Verlag

Elsevier Ltd

Erscheinungsjahr

1992

Link zum Volltext

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• A fermentation system was designed that utilizes n-paraffins as the main carbon/energy (C/E) source for penicillin biosynthesis, based upon the oxidation of phenyl-n-alkanes to phenyl acetic acid by Penicillium chrysogenum. n-Paraffins (component A) were found to be a suitable replacement for carbohydrate in penicillin fermentations when combined with an easily metabolizable substrate, triglycerol ester (component B). Some physico-chemical parameters (surface and interfacial tensions) of the oil mixture were determined to calculate the spreading force ( S), a contributing factor for adequate consumption rates of the non-aqueous liquid phase. Using a fed-batch-type fermentation system, key metabolic indicators, such as specific growth rate, residual n-paraffins, RO 2 and RCO 2, with its corresponding RQ, are presented. The applied technique in the fermentation conduction supports a penicillin synthesis rate comparable to the control.