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Modulation of neuropeptide FF release from rat spinal cord slices by glutamate. Involvement of NMDA receptors
Ist Teil von
European journal of pharmacology, 1994-12, Vol.271 (1), p.185-192
Ort / Verlag
Amsterdam: Elsevier B.V
Erscheinungsjahr
1994
Link zum Volltext
Quelle
Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)
Beschreibungen/Notizen
This study examined the effects of glutamate receptor agonists on the release of neuropeptide FF-like immunoreactivity from rat spinal dorsal half slices. Glutamate (10 μM) only induced release in Mg
2+-free medium enriched with glycine (1 μM) and with slight depolarization (15 mM K
+). This effect was abolished by the NMDA receptor antagonist, 2-amino-5-phosphonovalerate (100 μM), suggesting major participation of NMDA receptors. The quisqualate and metabotropic receptor agonists, α-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) and
trans-1-hydroxy-5-methylisoxazole-4-propionate (
t-ACPD) respectively, had no effect at 10 μM. In contrast, NMDA dose dependently stimulated neuropeptide FF release, even in the presence of the Na
+ channel blocker, tetrodotoxin (1 μM), suggesting that NMDA receptors involved in the release of neuropeptide FF are mainly located on nerve terminals. The NMDA receptor antagonists, 2-amino-5-phosphonovalerate or (+)-5-
methyl-10−11-
dihydro-5
H-dibenzo [
a,
d]cyclohepten-5,10-imine (MK-801) (100 μM), blocked the 10 μM NMDA effect. Furthermore, neuropeptide FF-like material inhibited binding of [
125I]Y8Fa, a radioiodinated analog of neuropeptide FF, to spinal membranes, suggesting physiological relevance of NMDA-induced release. Taken together, these results suggest a relationship between neuropeptide FF and NMDA receptors in the spinal cord.