Details

Autor(en) / Beteiligte

• Yeh, Tsung‐Han
• Huang, Shu‐Yu
• Lan, Wan‐Yu
• Liaw, Gwo‐Jen
• Yu, Jenn‐Yah

Titel

Modulation of cell morphogenesis by tousled‐like kinase in the Drosophila follicle cell

Ist Teil von

• Developmental dynamics, 2015-07, Vol.244 (7), p.852-865

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background: Tousled‐like kinase (Tlk) is a conserved serine/threonine kinase regulating DNA replication, chromatin assembly, and DNA repair. Previous studies have suggested that Tlk is involved in cell morphogenesis in vitro. In addition, tlk genetically interact with Rho1, which encodes a key regulator of the cytoskeleton. However, whether Tlk plays a physiological role in cell morphogenesis and cytoskeleton rearrangement remains unknown. Results: In tlk mutant follicle cells, area of the apical domain was reduced. The density of microtubules was increased in tlk mutant cells. The density of actin filaments was increased in the apical region and decreased in the basal region. Because area of the apical domain was reduced, we examined the levels of proteins located in the apical region by using immunofluorescence. The fluorescence intensities of two adherens junction proteins Armadillo (Arm) and DE‐cadherin (DE‐cad), atypical protein kinase C (aPKC), and Notch, were all increased in tlk mutant cells. The basolateral localized Discs large (Dlg) shifted apically in tlk mutant cells. Conclusions: Increase of protein densities in the apical region might be resulted from disruption of the cytoskeleton and shrinkage of the apical domain. Together, these data suggest a novel role of Tlk in maintaining cell morphology, possibly through modulating the cytoskeleton. Developmental Dynamics 244:852–865, 2015. © 2015 Wiley Periodicals, Inc. Key Findings The apical domain of tlk‐/‐ follicle cells is shrunken. The density of microtubules is increased in tlk‐/‐ cells. The level of actin filaments is increased in the apical and decreased in the basal region of tlk‐/‐ cells. The level of aPKC, DE‐cad and Arm is increased in the apical region of tlk‐/‐ cells. Dlg is re‐distributed towards the apical in tlk‐/‐ cells. Inactivation of Notch signaling is delayed in tlk‐/‐ cells.