Details

Autor(en) / Beteiligte

• Wenning, Leonie
• Yu, Tao
• David, Florian
• Nielsen, Jens
• Siewers, Verena

Titel

Establishing very long‐chain fatty alcohol and wax ester biosynthesis in Saccharomyces cerevisiae

Ist Teil von

• Biotechnology and bioengineering, 2017-05, Vol.114 (5), p.1025-1035

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• ABSTRACT Wax esters (WEs) are neutral lipids and can be used for a broad range of commercial applications, including personal care products, lubricants, or coatings. They are synthesized by enzymatic reactions catalyzed by a fatty acyl reductase (FAR) and a wax ester synthase (WS). At present, commercially used WEs are mainly isolated from Simmondsia chinensis (jojoba), but the high extraction costs and limited harvest areas constrain their use. The use of FARs in combination with different WSs to achieve a synthesis of jojoba‐like WEs in bacteria and yeast has been reported previously, but the products were restricted to C28–C36 WEs. These rather short WEs make up only a very small percentage of the total WEs in natural jojoba oil. The synthesis of longer chain WEs (up to C44) in Saccharomyces cerevisiae has so far only been achieved after substrate feeding. Here we identified new routes for producing very long‐chain fatty alcohols (VLCFOHs) up to a chain length of C22 by heterologous expression of a FAR derived from Apis mellifera (AmFAR1) or Marinobacter aquaeolei VT8 (Maqu_2220) in S. cerevisiae and achieved maximum yields of 3.22 ± 0.36 mg/g cell dry weight (CDW) and 7.84 ± 3.09 mg/g CDW, respectively, after 48 h. Moreover, we enabled the synthesis of jojoba‐like WEs up to a chain length of C42, catalyzed by a combination of Maqu_2220 together with the WS from S. chinensis (SciWS) and the S. cerevisiae elongase Elo2p, with a maximum yield of 12.24 ± 3.35 mg/g CDW after 48 h. Biotechnol. Bioeng. 2017;114: 1025–1035. © 2016 Wiley Periodicals, Inc. In this study a new synthesis route for very long‐chain fatty alcohols (C18–C22) and very long‐chain wax esters (C30–C42) in the yeast Saccharomyces cerevisiae was established. This was achieved by expression of heterologous genes, including the fatty acyl‐CoA reductases (FAR) from Apis mellifera (AmFAR) and Marinobacter aquaeolei VT8 (MaFAldhR = Maqu_2220) as well as the wax ester synthase (WS) from Simmondsia chinensis (SciWS) in a S. cerevisiae background strain containing a deletion of the ELO3 gene and a constitutive active ACC1 gene (ACC1**).