Details

Autor(en) / Beteiligte

• Westphal, Ines
• Jedelhauser, Claudia
• Liebsch, Gregor
• Wilhelmi, Arnd
• Aszodi, Attila
• Schieker, Matthias

Titel

Oxygen mapping: Probing a novel seeding strategy for bone tissue engineering

Ist Teil von

• Biotechnology and bioengineering, 2017-04, Vol.114 (4), p.894-902

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Wiley-Blackwell Full Collection

Beschreibungen/Notizen

• ABSTRACT Bone tissue engineering (BTE) utilizing biomaterial scaffolds and human mesenchymal stem cells (hMSCs) is a promising approach for the treatment of bone defects. The quality of engineered tissue is crucially affected by numerous parameters including cell density and the oxygen supply. In this study, a novel oxygen‐imaging sensor was introduced to monitor the oxygen distribution in three dimensional (3D) scaffolds in order to analyze a new cell‐seeding strategy. Immortalized hMSCs, pre‐cultured in a monolayer for 30–40% or 70–80% confluence, were used to seed demineralized bone matrix (DBM) scaffolds. Real‐time measurements of oxygen consumption in vitro were simultaneously performed by the novel planar sensor and a conventional needle‐type sensor over 24 h. Recorded oxygen maps of the novel planar sensor revealed that scaffolds, seeded with hMSCs harvested at lower densities (30–40% confluence), exhibited rapid exponential oxygen consumption profile. In contrast, harvesting cells at higher densities (70–80% confluence) resulted in a very slow, almost linear, oxygen decrease due to gradual achieving the stationary growth phase. In conclusion, it could be shown that not only the seeding density on a scaffold, but also the cell density at the time point of harvest is of major importance for BTE. The new cell seeding strategy of harvested MSCs at low density during its log phase could be a useful strategy for an early in vivo implantation of cell‐seeded scaffolds after a shorter in vitro culture period. Furthermore, the novel oxygen imaging sensor enables a continuous, two‐dimensional, quick and convenient to handle oxygen mapping for the development and optimization of tissue engineered scaffolds. Biotechnol. Bioeng. 2017;114: 894–902. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc. Oxygen mapping was introduced in bone tissue engineering (BTE) in order to analyze a novel cell‐seeding strategy. To study the influence of cell density at the time of harvest, oxygen consumption was analyzed in 3D‐scaffolds seeded with MSCs harvested at low (30–40%) and high confluence (70–80%). Oxygen maps revealed that scaffolds, seeded with cells harvested at low density, exhibited rapidly exponential oxygen consumption. In contrast, harvesting cells at higher density resulted in very slow, almost linear oxygen decrease. Authors have shown that not only the final seeding density but also the cell density at the time of harvest is important for BTE.