Details

Autor(en) / Beteiligte

• Oster, Stefan
• Daus, Andreas W.
• Erbes, Christian
• Goldhammer, Michael
• Bochtler, Ulrich
• Thielemann, Christiane

Titel

Long-term electromagnetic exposure of developing neuronal networks: A flexible experimental setup

Ist Teil von

• Bioelectromagnetics, 2016-05, Vol.37 (4), p.264-278

Ort / Verlag

United States: Blackwell Publishing Ltd

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• Neuronal networks in vitro are considered one of the most promising targets of research to assess potential electromagnetic field induced effects on neuronal functionality. A few exposure studies revealed there is currently no evidence of any adverse health effects caused by weak electromagnetic fields. Nevertheless, some published results are inconsistent. Particularly, doubts have been raised regarding possible athermal biological effects in the young brain during neuronal development. Therefore, we developed and characterized a flexible experimental setup based on a transverse electromagnetic waveguide, allowing controlled, reproducible exposure of developing neuronal networks in vitro. Measurement of S‐parameters confirmed very good performance of the Stripline in the band of 800–1000 MHz. Simulations suggested a flexible positioning of cell culture dishes throughout a large exposure area, as specific absorption rate values were quite independent of their position (361.7 ± 11.4 mW/kg) at 1 W, 900 MHz. During exposure, thermal drift inside cellular medium did not exceed 0.1 K. Embryonic rat cortical neurons were cultivated on microelectrode array chips to non‐invasively assess electrophysiological properties of electrogenic networks. Measurements were taken for several weeks, which attest to the experimental setup being a reliable system for long‐term studies on developing neuronal tissue. Bioelectromagnetics. 37:264–278, 2016. © 2016 Wiley Periodicals, Inc.