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The Phytase from Antarctic Bacterial Isolate, pseudomonas sp. JPK1 as a Potential Tool for Animal Agriculture to Reduce Manure Phosphorus Excretion
Ist Teil von
African journal of agricultural research, 2011-03, Vol.6 (6), p.1398-1406
Ort / Verlag
Nigeria: Academic Journals
Erscheinungsjahr
2011
Link zum Volltext
Quelle
EZB Free E-Journals
Beschreibungen/Notizen
Intracellular phytase activity was found in the Antarctic bacterial isolate, JPK1. Based on 16S rRNA gene sequence analysis, the strain was related to Pseudomonas sp. The optimal activity of JPK1 phytase occurred at 50°C and pH 5.0 to 5.5. The enzyme was highly specific for phytate with little or no other phosphate conjugates. Enzyme activity was strongly inhibited by Cu2+ and Zn2+, and completely inactivated by sodium dodecyl sulfate. The enzyme effectively liberated inorganic phosphate from wheat bran, a main feedstuff with high phytate content. The enzyme may be a good candidate for use as an environmental-friendly feed additive to enhance the nutritive value of phytate and reduce phosphorus pollution. [PUBLICATION ABSTRACT]