Details

Autor(en) / Beteiligte

• Tubbs, Raymond R.,
• Stoler, Mark H

Titel

Cell and tissue based molecular pathology [electronic resource]

Auflage

1st ed

Beschreibungen/Notizen

• Description based upon print version of record.
• Includes bibliographical references and index.
• Front cover; Cell and Tissue Based Molecular Pathology; Copyright page; Contributors; Foreword; Preface; Table of Contents; SECTION I. MOLECULAR PRINCIPLES; Chapter 1. Molecular Biology Basics for the Pathologist; INTRODUCTION; THE HUMAN GENOME; GENE STRUCTURE AND FUNCTION; Chapter 2. Quality Assurance of Molecular Assays; INTRODUCTION; DEVELOPMENT OF QUALITY ASSURANCE STANDARDS; EXAMPLES OF SPECIFIC QUALITY ASSURANCE GUIDELINES FOR TISSUE-BASED MOLECULAR ASSAYS; POLYMERASE CHAIN REACTION CONTAINMENT; CONCLUSION; SECTION II. MOLECULAR TOOLS AND TECHNIQUES
• Chapter 3. Overview of Molecular Diagnostic Techniques and InstrumentationINTRODUCTION; GENERAL METHODOLOGIES; SPECIFIC METHODOLOGIES; POLYMERASE CHAIN REACTION VARIATIONS; IN VITRO NUCLEIC ACID AMPLIFICATION ALTERNATIVES TO POLYMERASE CHAIN REACTION; SIGNAL AMPLIFICATION TECHNOLOGIES; Chapter 4. Conventional and Real-Time Polymerase Chain Reaction; INTRODUCTION; PRINCIPLES OF POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION: COMBINING AMPLIFICATION WITH DETECTION; APPLICATIONS; CONCLUSION; Chapter 5. Loss of Heterozygosity; PRINCIPLES OF LOSS OF HETEROZYGOSITY; CONCLUSION
• Chapter 6. Direct Genome Sequencing in Diagnostic PathologyINTRODUCTION; BASIS OF DIRECT DNA SEQUENCING FOR MOLECULAR DIAGNOSTICS; BASICS OF DNA SEQUENCING; DNA SEQUENCING BY CHAIN TERMINATION; RECENT DEVELOPMENTS IN DNA SEQUENCING; CONCLUSION; Chapter 7. Array-based Comparative Genomic Hybridization in the Analysis of Genomic Alterations in Human Disease; INTRODUCTION; CLASSICAL CYTOGENETICS AND METAPHASE-BASED COMPARATIVE GENOMIC HYBRIDIZATION; BACTERIAL ARTIFICIAL CHROMOSOME ARRAYS AND OTHER ALTERNATIVES; CHALLENGES AND LIMITATIONS OF ARRAY-BASED COMPARATIVE GENOMIC HYBRIDIZATION
• NONONCOLOGIC APPLICATIONS OF ARRAY-BASED COMPARATIVE GENOMIC HYBRIDIZATIONFLUORESCENCE IN SITU HYBRIDIZATION IN VALIDATION OF ARRAY-BASED COMPARATIVE GENOMIC HYBRIDIZATION DISCOVERIES; CONCLUSION; Chapter 8. Expression Arrays: Discovery and Validation; INTRODUCTION; MICROARRAY PLATFORMS; PROCEDURES; DATA PROCESSING; UNSUPERVISED ANALYSIS; SUPERVISED ANALYSIS; EXPRESSION SIGNATURES; VALIDATION; CONCLUSION; Chapter 9. Epigenetics: DNA Hypermethylation in Cancer; INTRODUCTION; EPIGENETIC CONTROL OF GENE EXPRESSION IN NORMAL CELLS; PROTEINS THAT REGULATE DNA METHYLATION
• DNA METHYLATION, CHROMATIN STRUCTURE, AND GENE EXPRESSIONDNA METHYLATION IN CANCER; DNA METHYLATION AS A MOLECULAR BIOMARKER IN CANCER DETECTION; THE EPIGENOME AS A TARGET FOR CANCER CHEMOPREVENTION AND TREATMENT; DNA METHYLATION AS A PROGNOSTIC OR PREDICTIVE BIOMARKER FOR CANCER; DNA METHYLATION ANALYSIS TECHNIQUES AND CLINICAL APPLICATIONS; CONCLUSION; Chapter 10. Tissue Microarrays; INTRODUCTION; PRINCIPLES OF THE TECHNIQUE; CONCLUSION; Chapter 11. Fluorescence In Situ Hybridization; PRINCIPLES OF TECHNIQUE; ADVANTAGES; DISADVANTAGES; APPLICATIONS; CONCLUSION
• Chapter 12. Bright-Field In Situ Hybridization
• This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you're getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and
• English