Details

Autor(en) / Beteiligte

• Popp, Jürgen,
• Maximillian Almond, Laurence,

Titel

Ex-vivo and in-vivo optical molecular pathology

Auflage

1st ed

Ort / Verlag

Weinheim, Germany : Wiley Blackwell,

Erscheinungsjahr

2014

Beschreibungen/Notizen

• Description based upon print version of record.
• Includes bibliographical references at the end of each chapters and index.
• Ex-vivo and In-vivo Optical Molecular Pathology; Contents; List of Contributors; Preface; Chapter 1 Clinical Pathology; 1.1 Introduction; 1.2 Pathology as a Medical and Research Discipline; 1.3 Historical Perspectives; 1.4 Specimens; 1.4.1 Biopsies, Resections, and Cytology; 1.5 Conventional Diagnostic Methods in Pathology; 1.5.1 Cytology; 1.5.2 Histology; 1.5.3 Microscopy; 1.5.4 Intraoperative Assessment (Frozen Section Examination); 1.5.5 Why Pathologists Do Not Like Frozen Tissue Examination?; 1.5.6 Microdissection
• 1.6 Nonconventional (Ancillary) Diagnostic Methods in Pathology (Molecular Assessment of Tissues)1.6.1 In Situ Reactions; 1.6.1.1 Immunofluorescence (IF); 1.6.1.2 Immunohistochemistry (IHC); 1.6.1.3 Fluorescent In Situ Hybridization (FISH); 1.6.2 Non-In Situ Methods; 1.7 Summary of Major Terms in Clinical Pathology; 1.7.1 Injury and Adaptation; 1.7.2 Inflammation and Repair; 1.7.3 Neoplastic Diseases; 1.8 Limitations of Clinical and Diagnostic Pathology; 1.8.1 Prediction of Tumor Behavior; 1.8.2 Diagnosis of Tumor Origin in a Case with Metastatic Tumor Disease
• 1.8.3 Individualized Medicine and Targeted TherapyFurther Readings; Chapter 2 Clinical Endoscopy in Gastrointestinal Diseases; 2.1 Introduction; 2.2 White-Light Endoscopy; 2.3 Chromoendoscopy; 2.4 Virtual Chromoendoscopy; 2.5 Endomicroscopy and Endocytoscopy; 2.6 Endoscopic Spectroscopy; 2.6.1 Autofluorescence Imaging; 2.6.2 Raman Spectroscopy; 2.7 Perspectives and Conclusions; References; Chapter 3 Molecular Pathology via Infrared and Raman Spectral Imaging; 3.1 Introduction; 3.2 Background; 3.3 Methods; 3.3.1 Sample Preparation; 3.3.2 Collection of Hyperspectral Data Cubes
• 3.3.3 Data Preprocessing3.3.4 Presorting (Cluster Analysis); 3.3.5 Visual Imaging; 3.3.6 Annotation; 3.3.7 Diagnostic Data Analysis; 3.3.8 Visual Data Analysis: Factor Methods; 3.4 Results and Discussion; 3.4.1 IR-SHP: Detection of Metastases and Micro-Metastases in Lymph Nodes; 3.4.1.1 IR-SHP of Micro-Metastases in Lymph Nodes; 3.4.1.2 IR-SHP of Lymphocyte Activation in Lymph Nodes; 3.4.2 SHP of Squamous Cell Carcinoma and Adenocarcinomas; 3.4.2.1 Distinction of Different Cancer Types by IR-SHP; 3.4.2.2 Diagnosis of Lung SqCC and Lung ADC via IR-SHP
• 3.4.2.3 Diagnosis of Cervical SqCC and Cervical ADC via SHP3.4.3 Results from Infrared Spectral Cytopathology (SCP); 3.4.3.1 Background; 3.4.3.2 Design and Methods of the Preclinical Trial for Oral Screening; 3.4.3.3 SCP Results for the Oral Mucosa; 3.4.3.4 SCP Results for the Cervical Mucosa; 3.4.4 IR and Raman-SHP of Brain Metastases; 3.4.4.1 IR and Raman Spectra of Brain Tissue; 3.4.4.2 Identification of Tumor Margins; 3.4.4.3 Determination of Primary Tumor; 3.4.5 Raman-SCP of Circulating Tumor Cells; 3.4.5.1 Dried Cells; 3.4.5.2 Tweezed Cells in Buffer
• 3.4.5.3 Trapped Cells in Microfluidic Chips
• The result of a unique collaboration between clinicians and physicists, this book provides an unparalleled overview of a new generation of diagnostic tools in clinical pathology. The introductory three chapters cover the present status and limitations of currently used methods, followed by an outline of promising novel spectroscopy-based technologies either under development or recently available on the market. The input from both physicists developing these new methods as well as routine clinicians familiar with practical aspects and medical relevance guarantees that this practical work i
• English
• Description based on online resource; title from PDF title page (ebrary, viewed April 14, 2014).