Details

Autor(en) / Beteiligte

• Crocker, J
• Murray, Paul,

Titel

Molecular biology in cellular pathology

Beschreibungen/Notizen

• Completed rev. & updated version of: Molecular biology in histopathology, 1994.
• Includes bibliographical references and index.
• Molecular Biology in Cellular Pathology; Contents; Preface; Preface to Molecular Biology in Histopathology; List of Contributors; 1 Blotting Techniques: Methodology and Applications; 1.1 Introduction; 1.2 Blotting techniques; 1.3 References; 2 In-situ Hybridisation in Histopathology; 2.1 Introduction; 2.2 Experimental conditions; 2.3 Probes and labels; 2.4 Controls and pitfalls; 2.5 Double-labelling; 2.6 Increasing the sensitivity of ISH; 2.7 What we do in our laboratories; 2.8 Applications of ISH: examples; 2.9 Perspective; 2.10 References; 3 DNA Flow Cytometry; 3.1 Introduction
• 3.2 Definitions and terms3.3 Dye used for DNA analysis; 3.4 Sample preparation for DNA analysis; 3.5 Analysis of the DNA histogram; 3.6 Quality control; 3.7 Computer analysis of the DNA histogram; 3.8 Multiparametric measurement; 3.9 Acknowledgements; 3.10 References; 4 Interphase Cytogenetics; 4.1 Introduction; 4.2 Interphase cytogenetics; 4.3 Applications; 4.4 Conclusion; 4.5 References; 5 Oncogenes; 5.1 Introduction; 5.2 Identification of the oncogenes; 5.3 Functions of the proto-oncogenes; 5.4 Mechanism of oncogene activation; 5.5 Oncogenes in colorectal cancer
• 5.6 Oncogenes in breast cancer5.7 Oncogenes in lung cancer; 5.8 Oncogenes in haematological malignancies; 5.9 Other cancers; 5.10 Conclusion; 5.11 References; 6 Molecular and Immunological Aspects of Cell Proliferation; 6.1 The cell cycle and its importance in clinical pathology; 6.2 Molecular control of the cell cycle; 6.3 Cell cycle control; 6.4 The cell cycle and cancer; 6.5 Immunocytochemical markers of proliferating cells; 6.6 References; 6.7 Further Reading; 7 Interphase Nucleolar Organiser Regions in Tumour Pathology; 7.1 Introduction; 7.2 The AgNORs; 7.3 NOR silver-staining
• 7.4 Quantitative AgNOR analysis7.5 AgNORs as a parameter of the level of cell proliferation; 7.6 Application of the AgNOR technique to tumour pathology; 7.7 What future for AgNORs in tumour pathology?; 7.8 References; 8 Apoptosis and Cell Senescence; 8.1 Introduction; 8.2 Apoptosis; 8.3 Cell senescence; 8.4 Summary; 8.5 References; 9 The Polymerase Chain Reaction; 9.1 Introduction; 9.2 Principles; 9.3 Analysis of products; 9.4 RT-PCR; 9.5 Quantitative PCR; 9.6 DNA and RNA extraction; 9.7 Correlation of the PCR with morphology; 9.8 Problems; 9.9 Applications; 9.10 Diagnostic applications
• 9.11 Infectious diseases9.12 Identity; 9.13 The future; 9.14 References; 9.15 Online information; 10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell; 10.1 Introduction; 10.2 The principle of LCM; 10.3 Technical considerations; 10.4 Advantages and disadvantages of LCM; 10.5 Applications of LCM; 10.6 Future perspectives; 10.7 Acknowledgements; 10.8 References; 11 The In-situ Polymerase Chain Reaction; 11.1 Introduction; 11.2 Overview of the methodology; 11.3 In-cell PCR technologies; 11.4 In-cell amplification of DNA
• 11.5 Detection of amplicons
• The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and ma
• English