Details

Autor(en) / Beteiligte

• Tseng, Fan-Gang,
• Santra, Tuhin Subhra,

Titel

Handbook of single-cell technologies

Ort / Verlag

Singapore : Springer,

Erscheinungsjahr

[2022]

Link zum Volltext

• SpringerLink Books - AutoHoldings

Beschreibungen/Notizen

• Intro -- Preface -- Contents -- About the Editors -- Contributors -- Part I: Physical Methods for Single Cell Therapy and Analysis -- 1 Light-Induced Cellular Delivery and Analysis -- Introduction -- Light-Induced Intracellular Delivery from Bulk to Single-Cell Photoporation -- Direct Laser-Cell Interaction -- Indirect Laser-Cell Interaction -- Pulsed Laser Systems for Optoporation -- Nanosecond Laser -- Femtosecond Laser -- Modifications of the Method -- Applications of Single-Cell Surgery -- Conclusion -- References -- 2 Mechanoporation: Toward Single Cell Approaches -- Introduction -- Types of Mechanoporation -- Particle Bombardment -- Microinjection -- Nanoneedle AFM Tip -- Microinjection Using Microfluidic Platforms -- Parallel Delivery -- Oscillating Nanoneedle Array -- Pressure Driven Devices -- Hydrodynamic Effect -- Sonoporation -- Shear Flow Devices -- A Cone and Plate Shearing Device -- Microchannel-Based Devices -- Mechanical Confinement -- High-Throughput Parallel Mechanical Confinement Device -- Microfluidic Devices -- Constriction Channel-Based Delivery -- Cell Squeeze Platform -- DNA Transfection Via Mechanical and Electrical Cell Membrane Disruption -- Advantages and Limitations of Mechanoporation -- Future Prospects -- Summary -- References -- 3 Single-Cell Electroporation -- Introduction -- Brief History -- Operation Mechanism -- Challenges in Macroscale Electroporation -- Landscape Transformation -- Electroporation Theories -- Intracellular Molecular Transport -- Cell Membrane Resealing Dynamics -- Microscale Electroporation -- Patch-Clamp Based Techniques -- Patch-Free Techniques -- Feedback-Driven Approaches -- Conclusion and Future Outlook -- References -- 4 Microinjection for Single-Cell Analysis and Therapy -- Introduction -- Development of Microinjection -- History of Microinjection.
• Types of Cargos Used for Microinjection -- Types of Host Cells Used in Microinjection -- Instruments for Microinjection -- Microinjection for Single-Cell Analysis -- Injection for Analysis of Changes in Cells -- Transgenic Animal Production -- Modern Advancements for Increasing Efficiency -- Microinjection for Single-Cell Therapy -- Intracytoplasmic Sperm Injection for Treatment of Infertility -- Gene Therapy by Injection -- Microneedles for Therapeutic Uses -- Advantages -- Disadvantages -- Future Perspective and Conclusion -- References -- Part II: Fluidic System and Integration -- 5 Single-Cell Manipulation -- Introduction -- Flow Modeling of Hydrodynamic Cell Manipulation -- Fluidic Resistance Versus Electric Resistance -- Conservation of Mass and Energy Versus Kirchhoff´s Current Law and Voltage Law -- Division of Pressure and Flow Rate Versus Division of Voltage and Current -- Examples of Microfluidic Manipulation -- Single-Cell Trapping -- Co-culture of Single Cells -- Deforming, Sorting, and Separating Single Cells -- Integration with Other Methods -- Optical Integration -- Dielectrophoretic Integration -- Magnetic Integration -- Acoustic Integration -- Conclusion -- References -- 6 Single Cell Manipulation Using Macro-scale Actuator -- Introduction -- Transfer Function of Macro-to-Micro Manipulation (Mizoue et al. 2017) -- The Driving Mechanism -- Mechanical Model -- System Identification Through Experiments -- On-Chip Transmitter for Enhancing Manipulation Speed (Monzawa et al. 2015) -- Advantages of Using an Actuation Transmitter -- Mechanical Model and Theoretical Basis -- Experimental Validation on Manipulation with an Actuation Transmitter -- Recent Works and Applications -- Summary -- References -- 7 Inertial Microfluidics for Single-Cell Manipulation and Analysis -- Introduction -- Underlying Physics -- Inertial Migration.
• Dean Flow -- Viscoelasticity -- Guidelines for Designing Spiral Inertial Microfluidics -- Dimensionless Numbers -- Design Guidelines -- Cross-Sectional Dimensions -- Channel Length (Loop Number) -- Other Structural Parameters -- Other Functional Units -- Operational Parameters -- Improved Understandings on Spiral Inertial Microfluidics -- Application Guidelines for Spiral Inertial Microfluidics -- Focusing/Ordering -- Separation -- Concentration/Microfiltration -- Conclusion and Future Perspective -- References -- 8 Digital Microfluidics for Single Cell Manipulation and Analysis -- Introduction -- DMF Systems -- DMF Technology -- Electrowetting-on-Dielectric -- Electrodewetting -- Liquid Dielectrophoresis -- Dielectrophoresis -- Optoelectrowetting -- Optoelectronic Tweezers -- Magnetic Force -- Fabrication of DMF -- DMF Manipulation and Analysis of a Single Cell -- Adherent Cell -- Suspension Cell -- Long-Term Culture -- Related Applications of DMF -- Cell Sorting and Concentrating -- 3D Cell Culture -- Diagnosis and Clinical Application -- Conclusions and Future Outlook -- References -- 9 Single-Cell Separation -- Introduction -- Conventional Cell Separation Techniques -- Centrifugation -- Fluorescence-Activated Cell Sorting -- Magnetic-Activated Cell Sorting -- Laser Capture Microdissection -- Manual Cell Picking -- Microfluidic Single-Cell Separation Techniques -- Microfluidic Passive Separation Techniques -- Filter-Based Separation -- Deterministic Lateral Displacement -- Hydrodynamic Separation -- Non-inertial Hydrodynamic Separation -- Inertial Hydrodynamic Separation -- Microfluidic Active Separation Techniques -- Dielectrophoresis (DEP) -- Magnetic Separation -- Acoustophoresis -- Affinity-Based Separation -- Comparison Between Different Microfluidic Separation Techniques -- Conclusion -- References.
• 10 Technologies for Automated Single Cell Isolation -- Introduction -- Cell Samples -- Size and Morphology of Cells -- Sample Preparation: Single-Cell Suspensions -- Basic Considerations, Definitions, and Classifications for Single-Cell Isolation -- Automated Single-Cell Isolation Technologies -- Limiting Dilution -- Suitability for Microbial Cells -- Fluorescence-Activated Cell Sorting -- Suitability for Microbial Cells -- Single-Cell Dispensing (SCD) -- Suitability for Microbial Cells -- Microfluidic Single-Cell Isolation -- Hydrodynamic Trapping -- Vertical Trapping in Nanowells -- Suitability for Microbial Cells -- Droplet Microfluidics -- Single-Cell Manipulation (on Microfluidic Chips) by Directed External Forces -- Optical Tweezers (OT) -- Dielectrophoresis (DEP) -- Suitability for Microbial Cells -- Automated Micromanipulators -- Discussion and Conclusion -- References -- 11 Dual-Well Microfluidic Technique for Single Cell Isolation and Long-Term Clonal Culture -- Introduction -- Applications of Monoclonal Cell Culture -- Overview of Single Cell Isolation Methods for Clonal Culture -- The Design Concept of the Dual-Well Technique -- Fabrication of Dual-Well Device -- Fabrication of Master Molds by Photolithography Technology -- Molding PDMS Device with the Master Molds -- Dual-Well Device Preparation for Single Cell Isolation -- Cell Suspension Preparation for Single Cell Isolation with Dual-Well Device -- Single Cell Isolation and Clonal Culture with Dual-Well Device -- Culture Medium Replacement of the Dual-Well Device -- Discussion -- The Effect of Capture Well Depth on Single Cell Efficiency -- The Effect of Washing Flow Rate on Single Cell Efficiency -- The Effect of Cell Type on Single Cell Capture Efficiency -- The Effect of Device Flipping on Cell Transfer Efficiency.
• Single Cell-Derived Clonal Colonies and Stem Cell Differentiation in the Microwells of the Dual-Well Device -- Conclusion -- References -- 12 Single-Cell Cultivation Utilizing Microfluidic Systems -- Introduction -- Purpose of Single-Cell Cultivation -- Morphology -- Proliferation and Differentiation -- Migration -- Genomics, Transcriptomics, Proteomics, and Metabolomics -- Epigenomics -- Single-Cell Cultivation in Microfluidic Devices -- Cell Coculture -- Neural Coculture -- Coculture of Tumor Cells to Neighboring Cells -- Coculture of Neurons and Cancer Cells -- Molecule-Induced Cellular Behaviors -- Molecular Cue-Guided Neuron -- Molecule and Immune Cell Interaction -- Molecule and Microbe Interaction -- Regeneration -- Axotomy -- Stem Cell-Based Regeneration -- Further Limitations and Future Prospects -- Conclusion -- References -- 13 Integrated Microwell Array Technologies for Single Cell Analysis -- Introduction -- Microfabrication of a Microwell Array -- Material and Design Considerations -- Soft Photolithography for Fast Prototyping of Microwell Arrays -- Hydrophilic-in-Hydrophobic Microwells -- Polyethylene Glycol Microwells for Reduced Non-specific Adsorption -- Single Cell Docking -- Docking Strategies: Manual Administration of Cells -- Docking Strategies: Microfluidics-Assisted Administration of Cells -- Continuous Flow Microfluidics -- Digital Microfluidics -- Single Cell Manipulation -- Optical Manipulation -- Magnetic Manipulation -- Mechanical Manipulation -- Electric Manipulation -- Applications -- Single Cell Drug Screening -- Single Cell Omics -- Detection of Single Cell Secreted Products -- Other Applications -- Conclusion -- References -- 14 Micro- and Nanopore Technologies for Single-Cell Analysis -- Coulter Principle -- A History Behind the Invention of Coulter Principle -- Single-Cell Counting Mechanism.
• Single-Cell Analysis with Conventional Coulter Counters.
• Description based on print version record.