Details

Autor(en) / Beteiligte

• Kloet, E. R. de,
• Sutanto, Win,

Titel

Methods in neurosciences. Volume 22. Neurobiology of steroids

Ort / Verlag

San Diego, California : Academic Press,

Erscheinungsjahr

[1994]

Link zum Volltext

• Elsevier Books AutoHoldings

Beschreibungen/Notizen

• Description based upon print version of record.
• Includes bibliographical references and index.
• Cover image; Title page; Table of Contents; Inside Front Cover; Copyright page; Contributors to Volume 22; Preface; Methods in Neurosciences; Section I: Steroid Kinetics and Metabolism; Chapter 1: Steroid Hormone Transport through Blood-Brain Barrier: Methods and Concepts; Publisher Summary; Introduction; Acknowledgment; Chapter 2: Enhanced Delivery of Steroids to the Brain Using a Redox-Based Chemical Delivery System; Publisher Summary; Introduction; Redox Methods for Brain-Enhanced Delivery of Steroids; Synthesis of Brain-Enhanced Estradiol-Chemical Delivery System
• Distribution of Estradiol-Chemical Delivery SystemPharmacology; Concluding Remarks; Acknowledgments; Chapter 3: Neurosteroids: Biosynthesis and Function; Publisher Summary; Neurosteroids: A Brief History; Neurosteroid Metabolism in Brain; Mechanisms of Neurosteroid Action: Genomic Effects; Nongenomic, Membrane Receptor-Mediated Activities of Neurosteroids; Neurosteroid Physiology; Concluding Remarks; Acknowledgments; Chapter 4: Cytochrome P450 Enzymes in Brain; Publisher Summary; Introduction; Quantitation and Subcellular Distribution of Brain P450
• Detection of Specific Forms of P450 in Brain by Catalytic ActivitiesArachidonic Acid Metabolism; Immunological Detection of Brain P450; Brain P450 Purification and Characterization; Detection of P450 mRNAS in Brain; Induction of Brain P450; Human Brain P450; General Conclusions; Acknowledgments; Chapter 5: Methods for Estimating 11β-Hydroxysteroid Dehydrogenase Activity; Publisher Summary; Introduction; Methods of Detecting 11β-Hydroxysteroid Dehydrogenase Activity; Concluding Remarks; Section II: Steroid Receptors
• Chapter 6: Steroid Hormone Binding to Intracellular Receptors: In Vitro and in Vivo StudiesPublisher Summary; Introduction; Cell Nuclear Retention of Corticosteroids; Binding Assay for Cytosolic Corticosteroid Receptors; Chapter 7: Steroid Hormone Binding to Membrane Receptors; Publisher Summary; Introduction; Criteria for Physiologically Relevant Receptors; Methods; Characterization of [3H]Corticosterone Binding to Amphibian Brain Membranes; Concluding Remarks; Acknowledgments; Chapter 8: In Vitro Autoradiography for Steroid Receptors; Publisher Summary; Introduction; Estrogen Receptors
• Corticosteroid, Androgen, and Progestin ReceptorsConclusions and Prospectus; Acknowledgments; Chapter 9: Immunocytochemical Studies on Glucocorticoid Receptor; Publisher Summary; Monoclonal Antibodies Demonstrate Glucocorticoid Receptor in Rat Central Nervous System; Subcellular Distribution of Glucocorticoid Receptors; Glucocorticoid Receptors and Pain Mechanisms; Conclusions; Acknowledgments; Chapter 10: Confocal Scanning Laser Microscopy of Steroid Receptors in Brain; Publisher Summary; Introduction; Confocal Scanning Laser Microscopy
• Immunolabeling for Confocal Scanning Laser Microscopy: Special Requirements
• Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions.Key FeaturesComprehensive protocols included for the study of* Steroid kinetics and metabolism* Steroid receptors* Molecular and cellular effects of steroids* Steroid effects on integrated systems
• English
• Description based on online resource; title from PDF title page (ebrary, viewed August 20, 2014).