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Ancestral protein reconstruction: techniques and applications

Biological chemistry, 2016-01, Vol.397 (1), p.1

2016

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Molecular basis for the allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex

Nature communications, 2021-05, Vol.12 (1), p.2748-2748, Article 2748

2021

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Rosetta:MSF: a modular framework for multi-state computational protein design

PLoS computational biology, 2017-06, Vol.13 (6), p.e1005600-e1005600

2017

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Prediction of quaternary structure by analysis of hot spot residues in protein‐protein interfaces: the case of anthranilate phosphoribosyltransferases

Proteins, structure, function, and bioinformatics, 2019-10, Vol.87 (10), p.815-825

2019

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Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

BioTechniques, 2018-01, Vol.64 (1), p.24-26

2018

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Mapping key amino acid residues for the epimerase efficiency and stereospecificity of the sex pheromone biosynthetic short-chain dehydrogenases/reductases of Nasonia

Scientific reports, 2019-01, Vol.9 (1), p.330-330, Article 330

2019

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Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

Cell chemical biology, 2016-06, Vol.23 (6), p.709-715

2016

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Rekonstruktion urzeitlicher Proteine

Biospektrum, 2015-11, Vol.21 (7), p.712-714

2015

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Long-Term Persistence of Bi-functionality Contributes to the Robustness of Microbial Life through Exaptation

PLoS genetics, 2016-01, Vol.12 (1), p.e1005836-e1005836

2016

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Establishing wild-type levels of catalytic activity on natural and artificial (βα)₈-barrel protein scaffolds

Proceedings of the National Academy of Sciences - PNAS, 2009-03, Vol.106 (10), p.3704-3709

2009

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Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology

International journal of molecular sciences, 2019-10, Vol.20 (20), p.5106

2019

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Identification and Characterization of a Bacterial Glycerol-1-phosphate Dehydrogenase: Ni2+-Dependent AraM from Bacillus subtilis

Biochemistry (Easton), 2008-07, Vol.47 (28), p.7376-7384

2008

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Proteinstabilisierung durch gelenkte Evolution mit Faltungsreportern

Biospektrum, 2011, Vol.17 (1), p.35-37

2011

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Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design

Protein engineering, design and selection, 2015-06, Vol.28 (6), p.147-151

2015

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Mimicking Enzyme Evolution by Generating New (β α)8-Barrels from (β α)4-Half-Barrels

Proceedings of the National Academy of Sciences - PNAS, 2004-11, Vol.101 (47), p.16448-16453

2004

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Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

Scientific reports, 2016-10, Vol.6 (1), p.34697-34697, Article 34697

2016

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A comprehensive analysis of the geranylgeranylglyceryl phosphate synthase enzyme family identifies novel members and reveals mechanisms of substrate specificity and quaternary structure organization

Molecular microbiology, 2014-05, Vol.92 (4), p.885-899

2014

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Related (βα)8-Barrel Proteins in Histidine and Tryptophan Biosynthesis: A Paradigm to Study Enzyme Evolution

Chembiochem : a European journal of chemical biology, 2011-07, Vol.12 (10), p.1487-1494

2011

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Activation of a chimeric Rpb5/RpoH subunit using library selection

PloS one, 2014-01, Vol.9 (1), p.e87485

2014

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High-Resolution Crystal Structure of an Artificial (βα)8-Barrel Protein Designed from Identical Half-Barrels

Biochemistry (Easton), 2009-02, Vol.48 (6), p.1145-1147

2009

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Directed Evolution of a (β α )8-barrel Enzyme to Catalyze Related Reactions in Two Different Metabolic Pathways

Proceedings of the National Academy of Sciences - PNAS, 2000-08, Vol.97 (18), p.9925-9930

2000

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Analysis of allosteric communication in a multienzyme complex by ancestral sequence reconstruction

Proceedings of the National Academy of Sciences - PNAS, 2020-01, Vol.117 (1), p.346-354

2020

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Improving enzyme functional annotation by integrating in vitro and in silico approaches: The example of histidinol phosphate phosphatases

Protein science, 2024-02, Vol.33 (2), p.e4899-n/a

2024

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Structural and Functional Characterization of the Ureidoacrylate Amidohydrolase RutB from Escherichia coli

Biochemistry (Easton), 2023-02, Vol.62 (3), p.863-872

2023

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Experimental and computational analysis of the ancestry of an evolutionary young enzyme from histidine biosynthesis

Protein science, 2023-01, Vol.32 (1), p.e4536-n/a

2023

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Ancestral protein reconstruction: techniques and applications

Molecular basis for the allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex

Rosetta:MSF: a modular framework for multi-state computational protein design

Prediction of quaternary structure by analysis of hot spot residues in protein‐protein interfaces: the case of anthranilate phosphoribosyltransferases

Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

Mapping key amino acid residues for the epimerase efficiency and stereospecificity of the sex pheromone biosynthetic short-chain dehydrogenases/reductases of Nasonia

Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

Rekonstruktion urzeitlicher Proteine

Long-Term Persistence of Bi-functionality Contributes to the Robustness of Microbial Life through Exaptation

Establishing wild-type levels of catalytic activity on natural and artificial (βα)₈-barrel protein scaffolds

Light-Regulation of Tryptophan Synthase by Combining Protein Design and Enzymology

Identification and Characterization of a Bacterial Glycerol-1-phosphate Dehydrogenase: Ni2+-Dependent AraM from Bacillus subtilis

Proteinstabilisierung durch gelenkte Evolution mit Faltungsreportern

Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design

Mimicking Enzyme Evolution by Generating New (β α)8-Barrels from (β α)4-Half-Barrels

Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

A comprehensive analysis of the geranylgeranylglyceryl phosphate synthase enzyme family identifies novel members and reveals mechanisms of substrate specificity and quaternary structure organization

Related (βα)8-Barrel Proteins in Histidine and Tryptophan Biosynthesis: A Paradigm to Study Enzyme Evolution

Activation of a chimeric Rpb5/RpoH subunit using library selection

High-Resolution Crystal Structure of an Artificial (βα)8-Barrel Protein Designed from Identical Half-Barrels

Directed Evolution of a (β α )8-barrel Enzyme to Catalyze Related Reactions in Two Different Metabolic Pathways

Analysis of allosteric communication in a multienzyme complex by ancestral sequence reconstruction

Improving enzyme functional annotation by integrating in vitro and in silico approaches: The example of histidinol phosphate phosphatases

Structural and Functional Characterization of the Ureidoacrylate Amidohydrolase RutB from Escherichia coli

Experimental and computational analysis of the ancestry of an evolutionary young enzyme from histidine biosynthesis

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