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Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations

Cellular and molecular life sciences : CMLS, 2020-10, Vol.77 (19), p.3875-3884

2020

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Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express

Biotechnology progress, 2019-03, Vol.35 (2), p.e2772-n/a

2019

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Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost‐effective method for expression of complex molecules

Biotechnology journal, 2021-04, Vol.16 (4), p.e2000230-n/a

2021

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Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase‐mediated cassette exchange system

Biotechnology progress, 2021-07, Vol.37 (4), p.e3140-n/a

2021

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The new frontier in CHO cell line development: From random to targeted transgene integration technologies

Biotechnology advances, 2024-10, Vol.75, p.108402, Article 108402

2024

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Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position

Biotechnology progress, 2020-07, Vol.36 (4), p.e2967-n/a

2020

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SPEED-MODE cell line development (CLD): Reducing Chinese hamster ovary (CHO) CLD timelines via earlier suspension adaptation and maximizing time spent in the exponential growth phase

Biotechnology progress, 2024-05, p.e3479-e3479

2024

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Full-length dystrophin restoration via targeted exon integration by AAV-CRISPR in a humanized mouse model of Duchenne muscular dystrophy

Molecular therapy, 2021-11, Vol.29 (11), p.3243-3257

2021

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Genome Editing of Medaka

Methods in molecular biology (Clifton, N.J.), 2023, Vol.2637, p.325

2023

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A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis

Developmental biology, 2024-02, Vol.506, p.42-51

2024

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Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

Molecular therapy. Nucleic acids, 2024-12, Vol.35 (4), p.102320, Article 102320

2024

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Efficient Nuclease-Directed Integration of Lentivirus Vectors into the Human Ribosomal DNA Locus

Molecular therapy, 2020-08, Vol.28 (8), p.1858-1875

2020

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Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ Vectors

Molecular therapy, 2019-12, Vol.27 (12), p.2195-2212

2019

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Adenovirus vectors in hematopoietic stem cell genome editing

FEBS letters, 2019-12, Vol.593 (24), p.3623-3648

2019

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Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

ACS synthetic biology, 2018-09, Vol.7 (9), p.2148-2159

2018

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Improving the secretory capacity of CHO producer cells: The effect of controlled Blimp1 expression, a master transcription factor for plasma cells

Metabolic engineering, 2022-01, Vol.69, p.73-86

2022

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Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges

Journal of molecular biology, 2011-03, Vol.407 (2), p.193-221

2011

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Manufacturability and functionality assessment of different formats of T-cell engaging bispecific antibodies

mAbs, 2023, Vol.15 (1), p.2231129-2231129

2023

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Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells

ACS synthetic biology, 2020-09, Vol.9 (9), p.2546-2561

2020

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CRISPR‐interceded CHO cell line development approaches

Biotechnology and bioengineering, 2023-04, Vol.120 (4), p.865-902

2023

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CRISPR-Cas9-Mediated In Vivo Gene Integration at the Albumin Locus Recovers Hemostasis in Neonatal and Adult Hemophilia B Mice

Molecular therapy. Methods & clinical development, 2020-09, Vol.18, p.520-531

2020

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CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum

Fungal genetics and biology, 2019-05, Vol.126, p.17-24

2019

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Sleeping Beauty Transposon Insertions into Nucleolar DNA by an Engineered Transposase Localized in the Nucleolus

International journal of molecular sciences, 2023-10, Vol.24 (19), p.14978

2023

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Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration

eLife, 2022-06, Vol.11

2022

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Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations

Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express

Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost‐effective method for expression of complex molecules

Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase‐mediated cassette exchange system

The new frontier in CHO cell line development: From random to targeted transgene integration technologies

Maximizing antibody production in a targeted integration host by optimization of subunit gene dosage and position

SPEED-MODE cell line development (CLD): Reducing Chinese hamster ovary (CHO) CLD timelines via earlier suspension adaptation and maximizing time spent in the exponential growth phase

Full-length dystrophin restoration via targeted exon integration by AAV-CRISPR in a humanized mouse model of Duchenne muscular dystrophy

Genome Editing of Medaka

Efficient targeted integration directed by short homology in zebrafish and mammalian cells

A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis

Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

Efficient Nuclease-Directed Integration of Lentivirus Vectors into the Human Ribosomal DNA Locus

Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ Vectors

Adenovirus vectors in hematopoietic stem cell genome editing

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation

Improving the secretory capacity of CHO producer cells: The effect of controlled Blimp1 expression, a master transcription factor for plasma cells

Recombinase-Mediated Cassette Exchange (RMCE): Traditional Concepts and Current Challenges

Manufacturability and functionality assessment of different formats of T-cell engaging bispecific antibodies

Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells

CRISPR‐interceded CHO cell line development approaches

CRISPR-Cas9-Mediated In Vivo Gene Integration at the Albumin Locus Recovers Hemostasis in Neonatal and Adult Hemophilia B Mice

CRISPR/Cas9-mediated endogenous gene tagging in Fusarium oxysporum

Sleeping Beauty Transposon Insertions into Nucleolar DNA by an Engineered Transposase Localized in the Nucleolus

Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration

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