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Force and the α-C-terminal domains bias RNA polymerase recycling

Nature communications, 2024-08, Vol.15 (1), p.7520-10, Article 7520

2024

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OptoLacI: optogenetically engineered lactose operon repressor LacI responsive to light instead of IPTG

Nucleic acids research, 2024-07, Vol.52 (13), p.8003-8016

2024

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Control of DNA replication in vitro using a reversible replication barrier

Nature protocols, 2024-07, Vol.19 (7), p.1940-1983

2024

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Engineering and application of LacI mutants with stringent expressions

Microbial biotechnology, 2024-03, Vol.17 (3), p.e14427-n/a

2024

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A tuneable genetic switch for tight control of tac promoters in Escherichia coli boosts expression of synthetic injectisomes

Microbial biotechnology, 2024-01, Vol.17 (1), p.e14328-n/a

2024

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Simulated pressure changes in LacI suggest a link between hydration and functional conformational changes

Biophysical chemistry, 2024-01, Vol.304, p.107126-107126, Article 107126

2024

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Genetic switching by the Lac repressor is based on two-state Monod-Wyman-Changeux allostery

Proceedings of the National Academy of Sciences - PNAS, 2023-12, Vol.120 (49), p.e2311240120-e2311240120

2023

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Silencing Transcription from an Influenza Reverse Genetics Plasmid in E. coli Enhances Gene Stability

ACS synthetic biology, 2023-02, Vol.12 (2), p.432-445

2023

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Robustness of DNA looping across multiple cell divisions in individual bacteria

Proceedings of the National Academy of Sciences - PNAS, 2022-08, Vol.119 (33), p.e2200061119-e2200061119

2022

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Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops

Nucleic acids research, 2022-03, Vol.50 (5), p.2826-2835

2022

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T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Microbial cell factories, 2022-01, Vol.21 (1), p.13-13, Article 13

2022

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Sequence specificity in DNA binding is mainly governed by association

Science (American Association for the Advancement of Science), 2022-01, Vol.375 (6579), p.442-445

2022

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Switching off: The phenotypic transition to the uninduced state of the lactose uptake pathway

Biophysical journal, 2022-01, Vol.121 (2), p.183-192

2022

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Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor

PLoS genetics, 2021-09, Vol.17 (9), p.e1009039-e1009039

2021

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Modular and Single-Cell Sensors of Bacterial Ser/Thr Kinase Activity

ACS synthetic biology, 2021-09, Vol.10 (9), p.2340-2350

2021

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Rheostat functional outcomes occur when substitutions are introduced at nonconserved positions that diverge with speciation

Protein science, 2021-09, Vol.30 (9), p.1833-1853

2021

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Deep representation learning improves prediction of LacI-mediated transcriptional repression

Proceedings of the National Academy of Sciences - PNAS, 2021-07, Vol.118 (27), p.1

2021

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The genotype-phenotype landscape of an allosteric protein

Molecular systems biology, 2021-03, Vol.17 (3), p.e10179-n/a

2021

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Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites

Nucleic acids research, 2021-01, Vol.49 (2), p.1163-1172

2021

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Molecular mechanisms controlling fructose‐specific memory and catabolite repression in lactose metabolism by Streptococcus mutans

Molecular microbiology, 2021-01, Vol.115 (1), p.70-83

2021

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Characterization of Gene Repression by Designed Transcription Activator-like Effector Dimer Proteins

Biophysical journal, 2020-11, Vol.119 (10), p.2045-2054

2020

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DNA surface exploration and operator bypassing during target search

Nature (London), 2020-07, Vol.583 (7818), p.858-861

2020

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Evolution of a New Function by Fusion between Phage DNA and a Bacterial Gene

Molecular biology and evolution, 2020-05, Vol.37 (5), p.1329-1341

2020

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Tuning up Transcription Factors for Therapy

Molecules (Basel, Switzerland), 2020-04, Vol.25 (8), p.1902

Becskei, Attila

2020

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The lac repressor hinge helix in context: The effect of the DNA binding domain and symmetry

Biochimica et biophysica acta. General subjects, 2020-04, Vol.1864 (4), p.129538-129538, Article 129538

2020

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Force and the α-C-terminal domains bias RNA polymerase recycling

OptoLacI: optogenetically engineered lactose operon repressor LacI responsive to light instead of IPTG

Control of DNA replication in vitro using a reversible replication barrier

Engineering and application of LacI mutants with stringent expressions

A tuneable genetic switch for tight control of tac promoters in Escherichia coli boosts expression of synthetic injectisomes

Simulated pressure changes in LacI suggest a link between hydration and functional conformational changes

Genetic switching by the Lac repressor is based on two-state Monod-Wyman-Changeux allostery

Silencing Transcription from an Influenza Reverse Genetics Plasmid in E. coli Enhances Gene Stability

Robustness of DNA looping across multiple cell divisions in individual bacteria

Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops

T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Sequence specificity in DNA binding is mainly governed by association

Switching off: The phenotypic transition to the uninduced state of the lactose uptake pathway

Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor

Modular and Single-Cell Sensors of Bacterial Ser/Thr Kinase Activity

Rheostat functional outcomes occur when substitutions are introduced at nonconserved positions that diverge with speciation

Deep representation learning improves prediction of LacI-mediated transcriptional repression

The genotype-phenotype landscape of an allosteric protein

Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites

Molecular mechanisms controlling fructose‐specific memory and catabolite repression in lactose metabolism by Streptococcus mutans

Characterization of Gene Repression by Designed Transcription Activator-like Effector Dimer Proteins

DNA surface exploration and operator bypassing during target search

Evolution of a New Function by Fusion between Phage DNA and a Bacterial Gene

Tuning up Transcription Factors for Therapy

The lac repressor hinge helix in context: The effect of the DNA binding domain and symmetry

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