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Engineering an allosteric transcription factor to respond to new ligands

Nature methods, 2016-02, Vol.13 (2), p.177

2016

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Genetic switching by the Lac repressor is based on two-state Monod-Wyman-Changeux allostery

Proceedings of the National Academy of Sciences - PNAS, 2023-12, Vol.120 (49), p.e2311240120-e2311240120

2023

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Sequence specificity in DNA binding is mainly governed by association

Science (American Association for the Advancement of Science), 2022-01, Vol.375 (6579), p.442-445

2022

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The lac Repressor Displays Facilitated Diffusion in Living Cells

Science (American Association for the Advancement of Science), 2012-06, Vol.336 (6088), p.1595-1598

2012

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Cause and Consequence of Tethering a SubTAD to Different Nuclear Compartments

Molecular cell, 2016-02, Vol.61 (3), p.461-473

2016

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Promoter architecture dictates cell-to-cell variability in gene expression

Science (American Association for the Advancement of Science), 2014-12, Vol.346 (6216), p.1533-1536

2014

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Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites

Nucleic acids research, 2021-01, Vol.49 (2), p.1163

2021

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Switching off: The phenotypic transition to the uninduced state of the lactose uptake pathway

Biophysical journal, 2022-01, Vol.121 (2), p.183-192

2022

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Single-target regulators form a minor group of transcription factors in Escherichia coli K-12

Nucleic acids research, 2018-05, Vol.46 (8), p.3921

2018

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Rheostat functional outcomes occur when substitutions are introduced at nonconserved positions that diverge with speciation

Protein science, 2021-09, Vol.30 (9), p.1833-1853

2021

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Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops

Nucleic acids research, 2022-03, Vol.50 (5), p.2826

2022

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Direct measurement of transcription factor dissociation excludes a simple operator occupancy model for gene regulation

Nature genetics, 2014-04, Vol.46 (4), p.405-408

2014

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Deep representation learning improves prediction of LacI-mediated transcriptional repression

Proceedings of the National Academy of Sciences - PNAS, 2021-07, Vol.118 (27), p.1

2021

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Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression

Nucleic acids research, 2012-11, Vol.40 (21), p.11139

2012

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Robustness of DNA looping across multiple cell divisions in individual bacteria

Proceedings of the National Academy of Sciences - PNAS, 2022-08, Vol.119 (33), p.e2200061119-e2200061119

2022

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Creating Single-Copy Genetic Circuits

Molecular cell, 2016-07, Vol.63 (2), p.329-336

2016

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Electrochemical detection of Bisphenol A with high sensitivity and selectivity using recombinant protein-immobilized graphene electrodes

Biosensors & bioelectronics, 2015-09, Vol.71, p.214-221

2015

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DNA surface exploration and operator bypassing during target search

Nature (London), 2020-07, Vol.583 (7818), p.858-861

2020

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Transcription-factor binding and sliding on DNA studied using micro- and macroscopic models

Proceedings of the National Academy of Sciences - PNAS, 2013-12, Vol.110 (49), p.19796-19801

2013

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Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over‐looked physiological factors

Protein science, 2019-10, Vol.28 (10), p.1806-1818

2019

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The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing

PLoS biology, 2011-01, Vol.9 (1), p.e1000573-e1000573

2011

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Heterochromatin boundaries are hotspots for de novo kinetochore formation

Nature cell biology, 2011-07, Vol.13 (7), p.799

2011

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The genotype-phenotype landscape of an allosteric protein

Molecular systems biology, 2021-03, Vol.17 (3), p.e10179-n/a

2021

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Simulated pressure changes in LacI suggest a link between hydration and functional conformational changes

Biophysical chemistry, 2024-01, Vol.304, p.107126-107126, Article 107126

2024

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The lac repressor hinge helix in context: The effect of the DNA binding domain and symmetry

Biochimica et biophysica acta. General subjects, 2020-04, Vol.1864 (4), p.129538-129538, Article 129538

2020

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Engineering an allosteric transcription factor to respond to new ligands

Genetic switching by the Lac repressor is based on two-state Monod-Wyman-Changeux allostery

Sequence specificity in DNA binding is mainly governed by association

The lac Repressor Displays Facilitated Diffusion in Living Cells

Cause and Consequence of Tethering a SubTAD to Different Nuclear Compartments

Promoter architecture dictates cell-to-cell variability in gene expression

Programmable gene regulation for metabolic engineering using decoy transcription factor binding sites

Switching off: The phenotypic transition to the uninduced state of the lactose uptake pathway

Single-target regulators form a minor group of transcription factors in Escherichia coli K-12

Rheostat functional outcomes occur when substitutions are introduced at nonconserved positions that diverge with speciation

Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops

Direct measurement of transcription factor dissociation excludes a simple operator occupancy model for gene regulation

Deep representation learning improves prediction of LacI-mediated transcriptional repression

Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression

Robustness of DNA looping across multiple cell divisions in individual bacteria

Creating Single-Copy Genetic Circuits

Electrochemical detection of Bisphenol A with high sensitivity and selectivity using recombinant protein-immobilized graphene electrodes

DNA surface exploration and operator bypassing during target search

Transcription-factor binding and sliding on DNA studied using micro- and macroscopic models

Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over‐looked physiological factors

The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing

Heterochromatin boundaries are hotspots for de novo kinetochore formation

The genotype-phenotype landscape of an allosteric protein

Simulated pressure changes in LacI suggest a link between hydration and functional conformational changes

The lac repressor hinge helix in context: The effect of the DNA binding domain and symmetry

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