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Heparinase III with High Activity and Stability: Heterologous Expression, Biochemical Characterization, and Application in Depolymerization of Heparin

Journal of agricultural and food chemistry, 2024-02, Vol.72 (6), p.3045-3054

2024

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A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

PloS one, 2020-10, Vol.15 (10), p.e0240920-e0240920

2020

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Cloning, expression, and characterization of a novel heparinase I from Bacteroides eggerthii

Preparative biochemistry & biotechnology, 2020-05, Vol.50 (5), p.477-485

2020

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Influence of saccharide modifications on heparin lyase III substrate specificities

Glycobiology (Oxford), 2022-03, Vol.32 (3), p.208-217

2022

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Identification and characterization of a novel heparinase PCHepII from marine bacterium Puteibacter caeruleilacunae

Scientific reports, 2023-11, Vol.13 (1), p.20112-20112, Article 20112

2023

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NK cell heparanase controls tumor invasion and immune surveillance

The Journal of clinical investigation, 2017-07, Vol.127 (7), p.2777-2788

2017

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A novel fluorescent nanosensor for detection of heparin and heparinase based on CuInS2 quantum dots

Biosensors & bioelectronics, 2014-04, Vol.54, p.617-622

2014

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A Heparinase Sensor Based on a Ternary System of Hg 2+ -Heparin-Osmium Nanoparticles

Analytical chemistry (Washington), 2020-01, Vol.92 (1), p.1635-1642

2020

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Chemoenzymatic quantification for monitoring unpurified polysaccharide in rich medium

Applied microbiology and biotechnology, 2019-09, Vol.103 (18), p.7635-7645

2019

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Expression and characterization of an enhanced recombinant heparinase I with chitin binding domain

International journal of biological macromolecules, 2017-12, Vol.105 (Pt 1), p.1250-1258

2017

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Polycation-induced benzoperylene probe excimer formation and the ratiometric detection of heparin and heparinase

Biosensors & bioelectronics, 2016-01, Vol.75, p.404-410

2016

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Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection

Journal of biotechnology, 2013-01, Vol.163 (1), p.30-37

2013

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Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3-O-sulfoglucosamine residue

FEBS letters, 2011-08, Vol.585 (15), p.2461-2466

2011

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Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Analytical biochemistry, 2011-08, Vol.415 (1), p.59-66

2011

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Importance of IdoA and IdoA(2S) ring conformations in computational studies of glycosaminoglycan–protein interactions

Carbohydrate research, 2013-11, Vol.381, p.133-137

2013

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A new approach to glycan targeting: enzyme inhibition by oligosaccharide metalloshielding

Chemical communications (Cambridge, England), 2014-04, Vol.50 (31), p.4056-4058

2014

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Analysis of 3-O-sulfo group-containing heparin tetrasaccharides in heparin by liquid chromatography–mass spectrometry

Analytical biochemistry, 2014-06, Vol.455, p.3-9

2014

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Binding affinities of vascular endothelial growth factor (VEGF) for heparin-derived oligosaccharides

Bioscience reports, 2012-02, Vol.32 (1), p.71-81

2012

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Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli

Applied microbiology and biotechnology, 2013-04, Vol.97 (7), p.2907-2916

2013

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Heparin depolymerization by immobilized heparinase: A review

International journal of biological macromolecules, 2017-06, Vol.99, p.721-730

2017

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Isolation, Purification, and Characterization of Heparinase from Streptomyces variabilis MTCC 12266

Scientific reports, 2019-04, Vol.9 (1), p.6482-6482, Article 6482

2019

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The value of the thromboelastometry heparinase assay (HEPTEM) in cardiac surgery

Thrombosis and haemostasis, 2015-11, Vol.114 (5), p.1058-1063

2015

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Heparan Sulfates Regulate Axonal Excitability and Context Generalization through Ca 2+ /Calmodulin-Dependent Protein Kinase II

Cells (Basel, Switzerland), 2023-02, Vol.12 (5)

2023

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Immobilization alters heparin cleaving properties of heparinase I

Glycobiology (Oxford), 2017-11, Vol.27 (11), p.994-998

2017

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Apolipoprotein E mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors

PloS one, 2013-07, Vol.8 (7), p.e67982

2013

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Heparinase III with High Activity and Stability: Heterologous Expression, Biochemical Characterization, and Application in Depolymerization of Heparin

A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

Cloning, expression, and characterization of a novel heparinase I from Bacteroides eggerthii

Influence of saccharide modifications on heparin lyase III substrate specificities

Identification and characterization of a novel heparinase PCHepII from marine bacterium Puteibacter caeruleilacunae

NK cell heparanase controls tumor invasion and immune surveillance

A novel fluorescent nanosensor for detection of heparin and heparinase based on CuInS2 quantum dots

A Heparinase Sensor Based on a Ternary System of Hg 2+ -Heparin-Osmium Nanoparticles

Chemoenzymatic quantification for monitoring unpurified polysaccharide in rich medium

Expression and characterization of an enhanced recombinant heparinase I with chitin binding domain

Polycation-induced benzoperylene probe excimer formation and the ratiometric detection of heparin and heparinase

Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection

Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3-O-sulfoglucosamine residue

Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Importance of IdoA and IdoA(2S) ring conformations in computational studies of glycosaminoglycan–protein interactions

A new approach to glycan targeting: enzyme inhibition by oligosaccharide metalloshielding

Analysis of 3-O-sulfo group-containing heparin tetrasaccharides in heparin by liquid chromatography–mass spectrometry

Binding affinities of vascular endothelial growth factor (VEGF) for heparin-derived oligosaccharides

Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli

Heparin depolymerization by immobilized heparinase: A review

Isolation, Purification, and Characterization of Heparinase from Streptomyces variabilis MTCC 12266

The value of the thromboelastometry heparinase assay (HEPTEM) in cardiac surgery

Heparan Sulfates Regulate Axonal Excitability and Context Generalization through Ca 2+ /Calmodulin-Dependent Protein Kinase II

Immobilization alters heparin cleaving properties of heparinase I

Apolipoprotein E mediates attachment of clinical hepatitis C virus to hepatocytes by binding to cell surface heparan sulfate proteoglycan receptors

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