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Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice

Methods in molecular biology (Clifton, N.J.), 2020-01, Vol.2050, p.121

Takemoto, Tatsuya

2020

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Restriction of PD-1 function by cis -PD-L1/CD80 interactions is required for optimal T cell responses

Science (American Association for the Advancement of Science), 2019-05, Vol.364 (6440), p.558-566

2019

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Analysis of the singularity cells controlling the pattern formation in multi-cellular systems

Biophysics and Physicobiology, 2024, Vol.21(Supplemental), pp.e211001

2024

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PD-1 agonism by anti-CD80 inhibits T cell activation and alleviates autoimmunity

Nature immunology, 2022-03, Vol.23 (3), p.399-410

2022

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The Origin and Regulation of Neuromesodermal Progenitors (NMPs) in Embryos

Cells (Basel, Switzerland), 2024-03, Vol.13 (6), p.549

2024

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An inducible germ cell ablation chicken model for high-grade germline chimeras

Development (Cambridge), 2023-09, Vol.150 (18)

2023

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Axial stem cells deriving both posterior neural and mesodermal tissues during gastrulation

Current opinion in genetics & development, 2012-08, Vol.22 (4), p.374-380

2012

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Cas12a and MAD7, genome editing tools for breeding

Breeding Science, 2024, Vol.74(1), pp.22-31

2024

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Intercellular exchange of Wnt ligands reduces cell population heterogeneity during embryogenesis

Nature communications, 2023-04, Vol.14 (1), p.1924-1924, Article 1924

2023

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Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Scientific reports, 2015-06, Vol.5 (1), p.11315-11315, Article 11315

2015

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Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

PloS one, 2018-10, Vol.13 (10), p.e0206360-e0206360

2018

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Sox2 gene regulation via the D1 enhancer in embryonic neural tube and neural crest by the combined action of SOX2 and ZIC2

Genes to cells : devoted to molecular & cellular mechanisms, 2020-04, Vol.25 (4), p.242-256

2020

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Axial level‐dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate

Development, growth & differentiation, 2016-06, Vol.58 (5), p.427-436

2016

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Foxn1-β5t transcriptional axis controls CD8+ T-cell production in the thymus

Nature communications, 2017-02, Vol.8 (1), p.14419-14419, Article 14419

2017

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Study on Electron Emission from Phosphorus δ-Doped Si-QDs/Undoped Si-QDs Multiple-Stacked Structures

IEICE transactions on electronics, 2022-10, Vol.E105.C (10), p.610-615, Article 2021FUP0006

2022

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Mechanism of cell fate choice between neural and mesodermal development during early embryogenesis

Congenital anomalies, 2013-06, Vol.53 (2), p.61-66

Takemoto, Tatsuya

2013

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Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration

Nature communications, 2018-04, Vol.9 (1), p.1400-13, Article 1400

2018

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Functional analysis of a first hindlimb positioning enhancer via Gdf11 expression

Frontiers in cell and developmental biology, 2024, Vol.12, p.1302141-1302141

2024

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Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

Developmental biology, 2016-10, Vol.418 (1), p.1-9

2016

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Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1

Development (Cambridge), 2006-01, Vol.133 (2), p.297-306

2006

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Somatic cell reprogramming-free generation of genetically modified pigs

Science advances, 2016-09, Vol.2 (9), p.e1600803-e1600803

2016

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The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components

Biology open, 2020-02, Vol.9 (2)

2020

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Point mutagenesis in mouse reveals contrasting pathogenetic effects between MEN2B‐ and Hirschsprung disease‐associated missense mutations of the RET gene

Development, growth & differentiation, 2020-05, Vol.62 (4), p.214-222

2020

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Generation of PDX‐1 mutant porcine blastocysts by introducing CRISPR/Cas9‐system into porcine zygotes via electroporation

Animal science journal, 2019-01, Vol.90 (1), p.55-61

2019

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Hoxa13 regulates expression of common Hox target genes involved in cartilage development to coordinate the expansion of the autopodal anlage

Development, growth & differentiation, 2019-04, Vol.61 (3), p.228-251

2019

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Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice

Restriction of PD-1 function by cis -PD-L1/CD80 interactions is required for optimal T cell responses

Analysis of the singularity cells controlling the pattern formation in multi-cellular systems

PD-1 agonism by anti-CD80 inhibits T cell activation and alleviates autoimmunity

The Origin and Regulation of Neuromesodermal Progenitors (NMPs) in Embryos

An inducible germ cell ablation chicken model for high-grade germline chimeras

Axial stem cells deriving both posterior neural and mesodermal tissues during gastrulation

Cas12a and MAD7, genome editing tools for breeding

Intercellular exchange of Wnt ligands reduces cell population heterogeneity during embryogenesis

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation

Sox2 gene regulation via the D1 enhancer in embryonic neural tube and neural crest by the combined action of SOX2 and ZIC2

Axial level‐dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate

Foxn1-β5t transcriptional axis controls CD8+ T-cell production in the thymus

Study on Electron Emission from Phosphorus δ-Doped Si-QDs/Undoped Si-QDs Multiple-Stacked Structures

Mechanism of cell fate choice between neural and mesodermal development during early embryogenesis

Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration

Functional analysis of a first hindlimb positioning enhancer via Gdf11 expression

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1

Somatic cell reprogramming-free generation of genetically modified pigs

The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components

Point mutagenesis in mouse reveals contrasting pathogenetic effects between MEN2B‐ and Hirschsprung disease‐associated missense mutations of the RET gene

Generation of PDX‐1 mutant porcine blastocysts by introducing CRISPR/Cas9‐system into porcine zygotes via electroporation

Hoxa13 regulates expression of common Hox target genes involved in cartilage development to coordinate the expansion of the autopodal anlage

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