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Protein stability: computation, sequence statistics, and new experimental methods

Current opinion in structural biology, 2015-08, Vol.33, p.161-168

Magliery, Thomas J

2015

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High-Throughput Thermal Scanning: A General, Rapid Dye-Binding Thermal Shift Screen for Protein Engineering

Journal of the American Chemical Society, 2009-03, Vol.131 (11), p.3794-3795

2009

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Stabilizing Proteins from Sequence Statistics: The Interplay of Conservation and Correlation in Triosephosphate Isomerase Stability

Journal of molecular biology, 2012-07, Vol.420 (4-5), p.384-399

2012

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Protein stability by number: high-throughput and statistical approaches to one of protein science's most difficult problems

Current opinion in chemical biology, 2011-06, Vol.15 (3), p.443-451

2011

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Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation

Proceedings of the National Academy of Sciences - PNAS, 2013-03, Vol.110 (11), p.4261-4266

2013

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Sequence variation in ligand binding sites in proteins

BMC bioinformatics, 2005-09, Vol.6 (1), p.240-240, Article 240

2005

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Direct single-molecule observation of a protein living in two opposed native structures

Proceedings of the National Academy of Sciences - PNAS, 2009-06, Vol.106 (25), p.10153-10158

2009

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Beyond Consensus: Statistical Free Energies Reveal Hidden Interactions in the Design of a TPR Motif

Journal of molecular biology, 2004-10, Vol.343 (3), p.731-745

2004

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Characterization of an Italian founder mutation in the RING-finger domain of BRCA1

PloS one, 2014-02, Vol.9 (2), p.e86924-e86924

2014

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Editorial overview: From powerful tools to useful products: protein engineering after 35 years of directed evolution

Current opinion in structural biology, 2020-08, Vol.63, p.vi-viii

2020

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Re-engineering a split-GFP reassembly screen to examine RING-domain interactions between BARD1 and BRCA1 mutants observed in cancer patients

Molecular bioSystems, 2008, Vol.4 (6), p.599-605

2008

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Phylogenetic spread of sequence data affects fitness of SOD1 consensus enzymes: Insights from sequence statistics and structural analyses

Proteins, structure, function, and bioinformatics, 2018-06, Vol.86 (6), p.609-620

2018

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Phylogenetic spread of sequence data affects fitness of consensus enzymes: Insights from triosephosphate isomerase

Proteins, structure, function, and bioinformatics, 2020-02, Vol.88 (2), p.274-283

2020

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Insights into the mechanism of paraoxonase-1: Comparing the reactivity of the six-bladed β-propeller hydrolases

Biochemistry (Easton), 2018-12, Article acs.biochem.8b01115

2018

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Stoichiometry of triple-sieve tRNA editing complex ensures fidelity of aminoacyl-tRNA formation

Nucleic acids research, 2019-01, Vol.47 (2), p.929

2019

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Synthetic Approach to Stop-Codon Scanning Mutagenesis

Journal of the American Chemical Society, 2011-04, Vol.133 (16), p.6177-6186

2011

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Cysteine‐free rop: A four‐helix bundle core mutant has wild‐type stability and structure but dramatically different unfolding kinetics

Protein science, 2010-04, Vol.19 (4), p.670-679

2010

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Detecting Protein−Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism

Journal of the American Chemical Society, 2005-01, Vol.127 (1), p.146-157

2005

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Linker engineering in anti-TAG-72 antibody fragments optimizes biophysical properties, serum half-life, and high-specificity tumor imaging

The Journal of biological chemistry, 2018-06, Vol.293 (23), p.9030-9040

2018

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Engineering a tRNA and Aminoacyl-tRNA Synthetase for the Site-Specific Incorporation of Unnatural Amino Acids into Proteins in vivo

Proceedings of the National Academy of Sciences - PNAS, 1997-09, Vol.94 (19), p.10092-10097

1997

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Retraction of “Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity”

Journal of the American Chemical Society, 2018-02, Vol.140 (5), p.1976-1976

2018

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A 3E8.scFv.Cys-IR800 Conjugate Targeting TAG-72 in an Orthotopic Colorectal Cancer Model

Molecular imaging and biology, 2018-02, Vol.20 (1), p.47-54

2018

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Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity

Journal of the American Chemical Society, 2013-07, Vol.135 (27), p.9980-9983

2013

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Simplifying protein expression with ligation-free, traceless and tag-switching plasmids

Protein expression and purification, 2012-09, Vol.85 (1), p.9-17

2012

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An Antibody with a Variable-Region Coiled-Coil "Knob" Domain

Angewandte Chemie (International ed.), 2014-01, Vol.53 (1), p.132-135

International ed. in English, 2014

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Protein stability: computation, sequence statistics, and new experimental methods

High-Throughput Thermal Scanning: A General, Rapid Dye-Binding Thermal Shift Screen for Protein Engineering

Stabilizing Proteins from Sequence Statistics: The Interplay of Conservation and Correlation in Triosephosphate Isomerase Stability

Protein stability by number: high-throughput and statistical approaches to one of protein science's most difficult problems

Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation

Sequence variation in ligand binding sites in proteins

Direct single-molecule observation of a protein living in two opposed native structures

Beyond Consensus: Statistical Free Energies Reveal Hidden Interactions in the Design of a TPR Motif

Characterization of an Italian founder mutation in the RING-finger domain of BRCA1

Editorial overview: From powerful tools to useful products: protein engineering after 35 years of directed evolution

Re-engineering a split-GFP reassembly screen to examine RING-domain interactions between BARD1 and BRCA1 mutants observed in cancer patients

Phylogenetic spread of sequence data affects fitness of SOD1 consensus enzymes: Insights from sequence statistics and structural analyses

Phylogenetic spread of sequence data affects fitness of consensus enzymes: Insights from triosephosphate isomerase

Insights into the mechanism of paraoxonase-1: Comparing the reactivity of the six-bladed β-propeller hydrolases

Stoichiometry of triple-sieve tRNA editing complex ensures fidelity of aminoacyl-tRNA formation

Synthetic Approach to Stop-Codon Scanning Mutagenesis

Cysteine‐free rop: A four‐helix bundle core mutant has wild‐type stability and structure but dramatically different unfolding kinetics

Detecting Protein−Protein Interactions with a Green Fluorescent Protein Fragment Reassembly Trap: Scope and Mechanism

Linker engineering in anti-TAG-72 antibody fragments optimizes biophysical properties, serum half-life, and high-specificity tumor imaging

Engineering a tRNA and Aminoacyl-tRNA Synthetase for the Site-Specific Incorporation of Unnatural Amino Acids into Proteins in vivo

Retraction of “Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity”

A 3E8.scFv.Cys-IR800 Conjugate Targeting TAG-72 in an Orthotopic Colorectal Cancer Model

Mutational Analysis of 48G7 Reveals that Somatic Hypermutation Affects Both Antibody Stability and Binding Affinity

Simplifying protein expression with ligation-free, traceless and tag-switching plasmids

An Antibody with a Variable-Region Coiled-Coil "Knob" Domain

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