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Site-directed mutagenesis by polymerase chain reaction

Methods in molecular biology (Clifton, N.J.), 2002, Vol.182, p.85

2002

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PCR-Based Random Mutagenesis Method Using Spiked Oligonucleotides to Randomize Selected Parts of a Gene without any Wild-Type Background

BioTechniques, 1998-12, Vol.25 (6), p.958-965

1998

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Developing a programmed restriction endonuclease for highly specific DNA cleavage

Nucleic acids research, 2005-01, Vol.33 (22), p.7039

2005

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High-throughput purification of polyHis-tagged recombinant fusion proteins

Methods in molecular biology (Clifton, N.J.), 2003, Vol.205, p.199

2003

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On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target

Protein engineering, 2000-04, Vol.13 (4), p.275-281

2000

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Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII

Journal of molecular biology, 2003-08, Vol.331 (2), p.395

2003

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Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis

BioTechniques, 2000-08, Vol.29 (2), p.338-342

2000

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Structural and Biochemical Characterization of a New Mg2+ Binding Site Near Tyr94 in the Restriction Endonuclease PvuII

Journal of molecular biology, 2003-08, Vol.331 (2), p.395-406

2003

Volltextzugriff (PDF)

Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

Journal of molecular biology, 1998, Vol.283 (1), p.59-69

1998

Volltextzugriff (PDF)

Automated Purification of His 6 -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

BioTechniques, 2000-08, Vol.29 (2), p.338-342

2000

Volltextzugriff (PDF)

Automated Purification of His

BioTechniques, 2000-08, Vol.29 (2), p.338-342

2000

Volltextzugriff (PDF)

Structural and Biochemical Characterization of a New Mg 2+ Binding Site Near Tyr94 in the Restriction Endonuclease PvuII

Journal of molecular biology, 2003, Vol.331 (2), p.395-406

2003

Volltextzugriff (PDF)

Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site

European journal of biochemistry, 1998-11, Vol.258 (1), p.184-191

1998

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Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity

Directed Molecular Evolution of Proteins, 2002, p.309-327

2002

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Protein engineering of the restriction endonuclease Eco RV: Structure‐guided design of enzyme variants that recognize the base pairs flanking the recognition site

European journal of biochemistry, 1998-11, Vol.258 (1), p.184-191

1998

Volltextzugriff (PDF)

Protein engineering of the restriction endonuclease EcoRV

European journal of biochemistry, 1998-11, Vol.258 (1), p.184-191

1998

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EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodexynucleotides

Protein engineering, design and selection, 1996-11, Vol.9 (11), p.1005-1010

1996

Volltextzugriff (PDF)

Eco RV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodexynucleotides

Protein engineering, design and selection, 1996, Vol.9 (11), p.1005-1010

1996

Volltextzugriff (PDF)

EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodeoxynucleotides

Protein engineering, 1996-11, Vol.9 (11), p.1005-1010

1996

Volltextzugriff (PDF)

Adverse events during a disorder‐specific psychotherapy compared to a nonspecific psychotherapy in patients with chronic depression

Journal of clinical psychology, 2020-01, Vol.76 (1), p.7-19

2020

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Site-directed mutagenesis by polymerase chain reaction

PCR-Based Random Mutagenesis Method Using Spiked Oligonucleotides to Randomize Selected Parts of a Gene without any Wild-Type Background

Developing a programmed restriction endonuclease for highly specific DNA cleavage

High-throughput purification of polyHis-tagged recombinant fusion proteins

On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target

Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII

Automated purification of His6-tagged proteins allows exhaustive screening of libraries generated by random mutagenesis

Structural and Biochemical Characterization of a New Mg2+ Binding Site Near Tyr94 in the Restriction Endonuclease PvuII

Towards the design of rare cutting restriction endonucleases: using directed evolution to generate variants of EcoRV differing in their substrate specificity by two orders of magnitude

Automated Purification of His 6 -Tagged Proteins Allows Exhaustive Screening of Libraries Generated by Random Mutagenesis

Automated Purification of His

Structural and Biochemical Characterization of a New Mg 2+ Binding Site Near Tyr94 in the Restriction Endonuclease PvuII

Protein engineering of the restriction endonuclease EcoRV--structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site

Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity

Protein engineering of the restriction endonuclease Eco RV: Structure‐guided design of enzyme variants that recognize the base pairs flanking the recognition site

Protein engineering of the restriction endonuclease EcoRV

EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodexynucleotides

Eco RV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodexynucleotides

EcoRV-T94V: a mutant restriction endonuclease with an altered substrate specificity towards modified oligodeoxynucleotides

Adverse events during a disorder‐specific psychotherapy compared to a nonspecific psychotherapy in patients with chronic depression

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