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Strategy and success for the directed evolution of enzymes

Current opinion in structural biology, 2011-08, Vol.21 (4), p.473-480

Dalby, Paul A

2011

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Two strategies to engineer flexible loops for improved enzyme thermostability

Scientific reports, 2017-02, Vol.7 (1), p.41212-41212, Article 41212

2017

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Two-substrate enzyme engineering using small libraries that combine the substrate preferences from two different variant lineages

Scientific reports, 2024-01, Vol.14 (1), p.1287-1287, Article 1287

2024

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Engineering of enzymes using non-natural amino acids

Bioscience reports, 2022-08, Vol.42 (8), p.1

2022

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Virus lasers for biological detection

Nature communications, 2019-08, Vol.10 (1), p.3594-11, Article 3594

2019

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Crystal structures and molecular dynamics simulations of a humanised antibody fragment at acidic to basic pH

Scientific reports, 2023-09, Vol.13 (1), p.16281-16281, Article 16281

2023

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Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4

PloS one, 2024-04, Vol.19 (4), p.e0300964-e0300964

2024

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Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment

Molecular pharmaceutics, 2016-02, Vol.13 (2), p.307-319

2016

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Computational Design To Reduce Conformational Flexibility and Aggregation Rates of an Antibody Fab Fragment

Molecular pharmaceutics, 2018-08, Vol.15 (8), p.3079-3092

2018

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Functional and computational identification of a rescue mutation near the active site of an mRNA methyltransferase

Scientific reports, 2020-12, Vol.10 (1), p.21841-21841, Article 21841

2020

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Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents

Scientific reports, 2021-12, Vol.11 (1), p.23584-23584, Article 23584

2021

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Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

Proceedings of the National Academy of Sciences - PNAS, 2018-12, Vol.115 (52), p.E12192-E12200

2018

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Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Scientific reports, 2022-10, Vol.12 (1), p.17721-17721, Article 17721

2022

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Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

Proceedings of the National Academy of Sciences - PNAS, 2018-11, Vol.115 (47), p.E11043-E11052

2018

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Challenges in scaling up AAV-based gene therapy manufacturing

Trends in biotechnology (Regular ed.), 2023-10, Vol.41 (10), p.1268-1281

2023

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Hot spots-making directed evolution easier

Biotechnology advances, 2022-05, Vol.56, p.107926-107926, Article 107926

2022

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Identification of Protein-Excipient Interaction Hotspots Using Computational Approaches

International journal of molecular sciences, 2016-06, Vol.17 (6), p.853-853

2016

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An Evaluation of the Potential of NMR Spectroscopy and Computational Modelling Methods to Inform Biopharmaceutical Formulations

Pharmaceutics, 2018-09, Vol.10 (4), p.165

2018

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Comparison of the pH- and thermally-induced fluctuations of a therapeutic antibody Fab fragment by molecular dynamics simulation

Computational and structural biotechnology journal, 2021-01, Vol.19, p.2726-2741

2021

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Stability Convergence in Antibody Coformulations

Molecular pharmaceutics, 2022-11, Vol.19 (11), p.4098-4110

2022

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Machine learning reveals hidden stability code in protein native fluorescence

Computational and structural biotechnology journal, 2021-01, Vol.19, p.2750-2760

2021

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Lyophilization to enable distribution of ChAdOx1 and ChAdOx2 adenovirus-vectored vaccines without refrigeration

npj vaccines, 2023-06, Vol.8 (1), p.85-85, Article 85

2023

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Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy

Biochemical journal, 2010-11, Vol.432 (1), p.101-114

2010

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Stability enhancement in a mAb and Fab coformulation

Scientific reports, 2020-12, Vol.10 (1), p.21129-21129, Article 21129

2020

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Residue Effect-Guided Design: Engineering of S. Solfataricus β‑Glycosidase to Enhance Its Thermostability and Bioproduction of Ginsenoside Compound K

Journal of agricultural and food chemistry, 2023-11, Vol.71 (44), p.16669-16680

2023

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Strategy and success for the directed evolution of enzymes

Two strategies to engineer flexible loops for improved enzyme thermostability

Two-substrate enzyme engineering using small libraries that combine the substrate preferences from two different variant lineages

Engineering of enzymes using non-natural amino acids

Virus lasers for biological detection

Crystal structures and molecular dynamics simulations of a humanised antibody fragment at acidic to basic pH

Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4

Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment

Computational Design To Reduce Conformational Flexibility and Aggregation Rates of an Antibody Fab Fragment

Functional and computational identification of a rescue mutation near the active site of an mRNA methyltransferase

Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics

Challenges in scaling up AAV-based gene therapy manufacturing

Hot spots-making directed evolution easier

Identification of Protein-Excipient Interaction Hotspots Using Computational Approaches

An Evaluation of the Potential of NMR Spectroscopy and Computational Modelling Methods to Inform Biopharmaceutical Formulations

Comparison of the pH- and thermally-induced fluctuations of a therapeutic antibody Fab fragment by molecular dynamics simulation

Stability Convergence in Antibody Coformulations

Machine learning reveals hidden stability code in protein native fluorescence

Lyophilization to enable distribution of ChAdOx1 and ChAdOx2 adenovirus-vectored vaccines without refrigeration

Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy

Stability enhancement in a mAb and Fab coformulation

Residue Effect-Guided Design: Engineering of S. Solfataricus β‑Glycosidase to Enhance Its Thermostability and Bioproduction of Ginsenoside Compound K

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