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Production of disulfide-bonded proteins in Escherichia coli

Protein expression and purification, 2012-03, Vol.82 (1), p.240-251

Berkmen, Mehmet

2012

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SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Microbial cell factories, 2012-05, Vol.11 (1), p.56-56, Article 753

2012

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Production of antibodies in SHuffle Escherichia coli strains

Methods in enzymology, 2021, Vol.659, p.105-144

2021

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Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation

Proceedings of the National Academy of Sciences - PNAS, 2008-08, Vol.105 (33), p.11933-11938

2008

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Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions

Applied microbiology and biotechnology, 2020-11, Vol.104 (22), p.9693-9706

2020

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Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Nature communications, 2023-06, Vol.14 (1), p.3514-3514, Article 3514

2023

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Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

Nature communications, 2015-08, Vol.6 (1), p.8072-8072, Article 8072

2015

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SAS: Split antibiotic selection for identifying chaperones that improve protein solubility

Heliyon, 2024-03, Vol.10 (5), p.e26996-e26996, Article e26996

2024

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The Nonconsecutive Disulfide Bond of Escherichia coli Phytase (AppA) Renders It Dependent on the Protein-disulfide Isomerase, DsbC

The Journal of biological chemistry, 2005-03, Vol.280 (12), p.11387-11394

2005

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Production of Disulfide‐Bonded Proteins in Escherichia coli

Current protocols in molecular biology (Print), 2014-10, Vol.108 (1), p.16.1B.1-16.1B.21

2014

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Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote

Redox biology, 2019-09, Vol.26, p.101280-101280, Article 101280

2019

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Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata

Frontiers in molecular biosciences, 2021-12, Vol.8, p.734154-734154

2021

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The DsbA Signal Sequence Directs Efficient, Cotranslational Export of Passenger Proteins to the Escherichia coli Periplasm via the Signal Recognition Particle Pathway

Journal of Bacteriology, 2003-10, Vol.185 (19), p.5706-5713

2003

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TrbB from Conjugative Plasmid F Is a Structurally Distinct Disulfide Isomerase That Requires DsbD for Redox State Maintenance

Journal of Bacteriology, 2011-09, Vol.193 (18), p.4588-4597

2011

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Bridging the gap with bacterial art

FEMS microbiology letters, 2023-01, Vol.370

2023

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Production and applications of fluorobody from redox-engineered Escherichia coli

Applied microbiology and biotechnology, 2023-03, Vol.107 (5-6), p.1959-1970

2023

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Correction to: Production and applications of fluorobody from redox-engineered Escherichia coli

Applied microbiology and biotechnology, 2023-05, Vol.107 (9), p.3129-3129

2023

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Aeropyrum pernix membrane topology of protein VKOR promotes protein disulfide bond formation in two subcellular compartments

Microbiology (Society for General Microbiology), 2017-12, Vol.163 (12), p.1864

2017

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Use of the SHuffle Strains in Production of Proteins

Current Protocols in Protein Science, 2016-08, Vol.85 (1), p.5.26.1-5.26.21

2016

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A useful epitope tag derived from maltose binding protein

Protein science, 2021-06, Vol.30 (6), p.1235-1246

2021

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A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

Nature chemical biology, 2017-09, Vol.13 (9), p.1022

2017

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Visualizing Bacterial Colony Morphologies Using Time-Lapse Imaging Chamber MOCHA

Journal of bacteriology, 2018-01, Vol.200 (2)

2018

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Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli

The Journal of biological chemistry, 2019-11, Vol.294 (48), p.18046-18056

2019

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Functional metagenomics of the thioredoxin superfamily

The Journal of biological chemistry, 2021-01, Vol.296, p.100247, Article 100247

2021

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Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli

Protein expression and purification, 2019-01, Vol.153, p.7-17

2019

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Production of disulfide-bonded proteins in Escherichia coli

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

Production of antibodies in SHuffle Escherichia coli strains

Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation

Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions

Isolation of full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of Escherichia coli

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria

SAS: Split antibiotic selection for identifying chaperones that improve protein solubility

The Nonconsecutive Disulfide Bond of Escherichia coli Phytase (AppA) Renders It Dependent on the Protein-disulfide Isomerase, DsbC

Production of Disulfide‐Bonded Proteins in Escherichia coli

Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote

Insertion Specificity of the hATx-6 Transposase of Hydra magnipapillata

The DsbA Signal Sequence Directs Efficient, Cotranslational Export of Passenger Proteins to the Escherichia coli Periplasm via the Signal Recognition Particle Pathway

TrbB from Conjugative Plasmid F Is a Structurally Distinct Disulfide Isomerase That Requires DsbD for Redox State Maintenance

Bridging the gap with bacterial art

Production and applications of fluorobody from redox-engineered Escherichia coli

Correction to: Production and applications of fluorobody from redox-engineered Escherichia coli

Aeropyrum pernix membrane topology of protein VKOR promotes protein disulfide bond formation in two subcellular compartments

Use of the SHuffle Strains in Production of Proteins

A useful epitope tag derived from maltose binding protein

A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

Visualizing Bacterial Colony Morphologies Using Time-Lapse Imaging Chamber MOCHA

Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli

Functional metagenomics of the thioredoxin superfamily

Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli

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