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RT-qPCR Testing of SARS-CoV-2: A Primer

International journal of molecular sciences, 2020-04, Vol.21 (8), p.3004

2020

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Why the need for qPCR publication guidelines?—The case for MIQE

Methods (San Diego, Calif.), 2010-04, Vol.50 (4), p.217-226

Bustin, Stephen A.

2010

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Quantification of mRNA using real-time RT-PCR

Nature protocols, 2006-11, Vol.1 (3), p.1559-1582

2006

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The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Clinical chemistry (Baltimore, Md.), 2013-06, Vol.59 (6), p.892-902

2013

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Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis

Clinical science (1979), 2005-10, Vol.109 (4), p.365

2005

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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Clinical chemistry (Baltimore, Md.), 2009-04, Vol.55 (4), p.611-622

2009

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Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

Clinical chemistry (Baltimore, Md.), 2020-11, Vol.66 (11), p.1369-1372

2020

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MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

BMC molecular biology, 2010-09, Vol.11 (1), p.74-74

2010

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Validation of housekeeping genes for normalizing RNA expression in real-time PCR

BioTechniques, 2004-07, Vol.37 (1), p.112-119

2004

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Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

Expert review of molecular diagnostics, 2009-03, Vol.9 (2), p.187-197

2009

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SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations

Analytical biochemistry, 2006-04, Vol.351 (2), p.308-310

2006

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Proximity assays for sensitive quantification of proteins

Biomolecular detection and quantification, 2015-06, Vol.4, p.10-16

2015

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Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies

Analytical biochemistry, 2002-10, Vol.309 (2), p.293-300

2002

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The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Clinical chemistry (Baltimore, Md.), 2020-08, Vol.66 (8), p.1012-1029

Huggett, Jim F

2020

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Vitamin D Receptor (VDR) mRNA and VDR Protein Levels in Relation to Vitamin D Status, Insulin Secretory Capacity, and VDR Genotype in Bangladeshi Asians

Diabetes (New York, N.Y.), 2002-07, Vol.51 (7), p.2294-2300

2002

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The growth hormone–insulin-like growth factor-I axis and colorectal cancer

Trends in Molecular Medicine, 2001-10, Vol.7 (10), p.447-454

2001

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The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans

The journal of clinical endocrinology and metabolism, 2002-06, Vol.87 (6), p.2988-2991

2002

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The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors

The journal of clinical endocrinology and metabolism, 2001-02, Vol.86 (2), p.881-887

2001

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Evidence for a link between IGF-I and cancer

European journal of endocrinology, 2004-08, Vol.151 Suppl 1, p.S17

2004

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Differential Expression Patterns of the Insulin-Like Growth Factor 2 Gene in Human Colorectal Cancer

Tumor biology, 2004-01, Vol.25 (1-2), p.62-68

2004

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The Tissue Distribution of the mRNA of Ghrelin and Subtypes of Its Receptor, GHS-R, in Humans

The journal of clinical endocrinology and metabolism, 2002-06, Vol.87 (6), p.2988-2988

2002

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ERF-2, the human homologue of the murine Tis11d early response gene

Gene, 1995-01, Vol.152 (2), p.285-286

1995

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Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer

DNA and cell biology, 2001-06, Vol.20 (6), p.331

2001

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Molecular Biology of the Cell, Sixth Edition; ISBN: 9780815344643; and Molecular Biology of the Cell, Sixth Edition, The Problems Book; ISBN 9780815344537

International Journal of Molecular Sciences, 2015-11, Vol.16 (12), p.28123-28125

Bustin, Stephen

2015

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CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19

Scientific reports, 2020-12, Vol.10 (1), p.22214-22214, Article 22214

2020

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RT-qPCR Testing of SARS-CoV-2: A Primer

Why the need for qPCR publication guidelines?—The case for MIQE

Quantification of mRNA using real-time RT-PCR

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Validation of housekeeping genes for normalizing RNA expression in real-time PCR

Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations

Proximity assays for sensitive quantification of proteins

Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies

The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

Vitamin D Receptor (VDR) mRNA and VDR Protein Levels in Relation to Vitamin D Status, Insulin Secretory Capacity, and VDR Genotype in Bangladeshi Asians

The growth hormone–insulin-like growth factor-I axis and colorectal cancer

The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans

The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors

Evidence for a link between IGF-I and cancer

Differential Expression Patterns of the Insulin-Like Growth Factor 2 Gene in Human Colorectal Cancer

The Tissue Distribution of the mRNA of Ghrelin and Subtypes of Its Receptor, GHS-R, in Humans

ERF-2, the human homologue of the murine Tis11d early response gene

Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer

Molecular Biology of the Cell, Sixth Edition; ISBN: 9780815344643; and Molecular Biology of the Cell, Sixth Edition, The Problems Book; ISBN 9780815344537

CoV2-ID, a MIQE-compliant sub-20-min 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19

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