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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Chembiochem : a European journal of chemical biology, 2001-08, Vol.2 (7-8), p.517-523

2001

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Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

Analytica chimica acta, 2012-07, Vol.736, p.85-91

2012

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Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Journal of proteome research, 2005-11, Vol.4 (6), p.2137-2147

2005

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Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8+ T-cell response

Vaccine, 2016-06, Vol.34 (27), p.3093-3101

2016

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Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Protein engineering, design and selection, 2013-11, Vol.26 (11), p.725-734

2013

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Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

The Journal of biological chemistry, 2000-06, Vol.275 (24), p.18302-18310

2000

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Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

The Journal of biological chemistry, 2000-07, Vol.275 (28), p.21578-21586

2000

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Expression of recombinant alkaline phosphatase conjugates in Escherichia coli

Methods in molecular biology (Clifton, N.J.), 2004, Vol.267, p.101

2004

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Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

Journal of immunological methods, 1995-04, Vol.181 (2), p.177-186

1995

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Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

Bio/technology (New York, N.Y. 1983), 1993-05, Vol.11 (5), p.601-605

1993

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The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

The Journal of biological chemistry, 1997-09, Vol.272 (38), p.23775-23783

1997

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BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Protein expression and purification, 2004-11, Vol.38 (1), p.69-78

2004

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Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes

Toxicon (Oxford), 2000-03, Vol.38 (3), p.449-462

2000

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Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein 1 1Edited by R. Huber

Journal of molecular biology, 2000-03, Vol.296 (4), p.1017-1026

2000

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Direct expression in E.coli of a functionally active protein A–snake toxin fusion protein

Protein engineering, design and selection, 1989-11, Vol.3 (2), p.139-143

1989

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On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

FEBS letters, 1994-12, Vol.356 (2), p.153-158

1994

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A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

European Journal of Biochemistry, 1996-06, Vol.238 (3), p.653-660

1996

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A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

FEBS letters, 1986-11, Vol.208 (2), p.236-240

1986

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Mimicry between Receptors and Antibodies

The Journal of biological chemistry, 1996-12, Vol.271 (49), p.31345-31353

1996

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Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin

Journal of immunological methods, 1994-03, Vol.169 (2), p.205-211

1994

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Genetic Engineering of Snake Toxins

The Journal of biological chemistry, 1995-04, Vol.270 (16), p.9362-9369

1995

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Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues

The Journal of biological chemistry, 1995-04, Vol.270 (16), p.9362-9369

1995

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Neurotoxin-Specific Immunoglobulins Accelerate Dissociation of the Neurotoxin-Acetylcholine Receptor Complex

Science (American Association for the Advancement of Science), 1982-08, Vol.217 (4561), p.732-733

1982

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Complete amino-acid sequence of the β-subunit of VTX from venom of the stonefish ( Synanceia verrucosa) as identified from cDNA cloning experiments

Biochimica et biophysica acta, 1997-01, Vol.1337 (1), p.1-5

1997

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Production of recombinant notechis 11′2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli

European journal of biochemistry, 1993-03, Vol.212 (2), p.441-446

1993

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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8+ T-cell response

Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

Expression of recombinant alkaline phosphatase conjugates in Escherichia coli

Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes

Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein 1 1Edited by R. Huber

Direct expression in E.coli of a functionally active protein A–snake toxin fusion protein

On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

Mimicry between Receptors and Antibodies

Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin

Genetic Engineering of Snake Toxins

Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues

Neurotoxin-Specific Immunoglobulins Accelerate Dissociation of the Neurotoxin-Acetylcholine Receptor Complex

Complete amino-acid sequence of the β-subunit of VTX from venom of the stonefish ( Synanceia verrucosa) as identified from cDNA cloning experiments

Production of recombinant notechis 11′2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli

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