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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Chembiochem : a European journal of chemical biology, 2001-08, Vol.2 (7-8), p.517-523

2001

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Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Journal of proteome research, 2005-11, Vol.4 (6), p.2137-2147

2005

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Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Protein engineering, design and selection, 2013-11, Vol.26 (11), p.725-734

2013

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Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

The Journal of biological chemistry, 2000-06, Vol.275 (24), p.18302-18310

2000

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Recombinant single-chain Fv antibody fragment–alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization

Journal of immunological methods, 1999-07, Vol.227 (1), p.177-185

1999

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Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

The Journal of biological chemistry, 2000-07, Vol.275 (28), p.21578-21586

2000

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Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

Journal of immunological methods, 1995-04, Vol.181 (2), p.177-186

1995

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The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

The Journal of biological chemistry, 1997-09, Vol.272 (38), p.23775-23783

1997

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Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

Journal of immunological methods, 1996-10, Vol.197 (1), p.39-49

1996

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BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Protein expression and purification, 2004-11, Vol.38 (1), p.69-78

2004

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On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

FEBS letters, 1994-12, Vol.356 (2), p.153-158

1994

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A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

European Journal of Biochemistry, 1996-06, Vol.238 (3), p.653-660

1996

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A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

FEBS letters, 1986-11, Vol.208 (2), p.236-240

1986

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Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody

Protein engineering, 1994-07, Vol.7 (7), p.917-923

1994

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Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin

Journal of immunological methods, 1994-03, Vol.169 (2), p.205-211

1994

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Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues

The Journal of biological chemistry, 1995-04, Vol.270 (16), p.9362-9369

1995

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Complete amino-acid sequence of the β-subunit of VTX from venom of the stonefish ( Synanceia verrucosa) as identified from cDNA cloning experiments

Biochimica et biophysica acta, 1997-01, Vol.1337 (1), p.1-5

1997

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Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis

The Journal of biological chemistry, 1993-01, Vol.268 (2), p.909-916

1993

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Further evidence showing that neurotoxin-acetylcholine receptor dissociation is accelerated by monoclonal neurotoxin-specific immunoglobulin

Molecular immunology, 1985-01, Vol.22 (5), p.553-556

1985

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Precursors of vertebrate peptide antibiotics dermaseptin b and adenoregulin have extensive sequence identities with precursors of opioid peptides dermorphin, dermenkephalin, and deltorphins

The Journal of biological chemistry, 1994-07, Vol.269 (27), p.17847-17852

1994

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The interaction of neurotoxin derivatives with either acetylcholine receptor or a monoclonal antibody: an electron-spin-resonance study

European journal of biochemistry, 1984-01, Vol.140 (1), p.31-37

1984

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Mimicry between receptors and antibodies. Identification of snake toxin determinants recognized by the acetylcholine receptor and an acetylcholine receptor-mimicking monoclonal antibody

The Journal of biological chemistry, 1996-12, Vol.271 (49), p.31345-31353

1996

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Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein

Journal of molecular biology, 2000-03, Vol.296 (4), p.1017

2000

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Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual "rosary-type" organization

The Journal of biological chemistry, 1993-02, Vol.268 (5), p.3052-3055

1993

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Transformation of a non-enzymatic toxin into a toxoid by genetic engineering

Protein engineering, 1997-10, Vol.10 (10), p.1213-1220

1997

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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

Recombinant single-chain Fv antibody fragment–alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization

Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody

Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin

Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues

Complete amino-acid sequence of the β-subunit of VTX from venom of the stonefish ( Synanceia verrucosa) as identified from cDNA cloning experiments

Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis

Further evidence showing that neurotoxin-acetylcholine receptor dissociation is accelerated by monoclonal neurotoxin-specific immunoglobulin

Precursors of vertebrate peptide antibiotics dermaseptin b and adenoregulin have extensive sequence identities with precursors of opioid peptides dermorphin, dermenkephalin, and deltorphins

The interaction of neurotoxin derivatives with either acetylcholine receptor or a monoclonal antibody: an electron-spin-resonance study

Mimicry between receptors and antibodies. Identification of snake toxin determinants recognized by the acetylcholine receptor and an acetylcholine receptor-mimicking monoclonal antibody

Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein

Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual "rosary-type" organization

Transformation of a non-enzymatic toxin into a toxoid by genetic engineering

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