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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Chembiochem : a European journal of chemical biology, 2001-08, Vol.2 (7-8), p.517-523

2001

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Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

Analytica chimica acta, 2012-07, Vol.736, p.85-91

2012

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Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Journal of proteome research, 2005-11, Vol.4 (6), p.2137-2147

2005

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Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8+ T-cell response

Vaccine, 2016-06, Vol.34 (27), p.3093-3101

2016

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Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Protein engineering, design and selection, 2013-11, Vol.26 (11), p.725-734

2013

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Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

The Journal of biological chemistry, 2000-06, Vol.275 (24), p.18302-18310

2000

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Recombinant single-chain Fv antibody fragment–alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization

Journal of immunological methods, 1999-07, Vol.227 (1), p.177-185

1999

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Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

The Journal of biological chemistry, 2000-07, Vol.275 (28), p.21578-21586

2000

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Expression of recombinant alkaline phosphatase conjugates in Escherichia coli

Methods in molecular biology (Clifton, N.J.), 2004, Vol.267, p.101

2004

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Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

Journal of immunological methods, 1995-04, Vol.181 (2), p.177-186

1995

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Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

Bio/technology (New York, N.Y. 1983), 1993-05, Vol.11 (5), p.601-605

1993

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The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

The Journal of biological chemistry, 1997-09, Vol.272 (38), p.23775-23783

1997

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Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

Journal of immunological methods, 1996-10, Vol.197 (1), p.39-49

1996

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BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Protein expression and purification, 2004-11, Vol.38 (1), p.69-78

2004

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Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes

Toxicon (Oxford), 2000-03, Vol.38 (3), p.449-462

2000

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Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein 1 1Edited by R. Huber

Journal of molecular biology, 2000-03, Vol.296 (4), p.1017-1026

2000

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Direct expression in E.coli of a functionally active protein A–snake toxin fusion protein

Protein engineering, design and selection, 1989-11, Vol.3 (2), p.139-143

1989

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On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

FEBS letters, 1994-12, Vol.356 (2), p.153-158

1994

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Three-dimensional crystal structure of recombinant erabutoxin a at 2.0 Å resolution

FEBS letters, 1994-03, Vol.342 (1), p.12-14

1994

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A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

European Journal of Biochemistry, 1996-06, Vol.238 (3), p.653-660

1996

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A recombinant snake neurotoxin generated by chemical cleavage of a hybrid protein recovers full biological properties

FEBS letters, 1990-06, Vol.266 (1), p.87-90

1990

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Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence

Toxicon (Oxford), 1991, Vol.29 (4), p.516-520

1991

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A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

FEBS letters, 1986-11, Vol.208 (2), p.236-240

1986

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Mimicry between Receptors and Antibodies

The Journal of biological chemistry, 1996-12, Vol.271 (49), p.31345-31353

1996

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Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody

Protein engineering, 1994-07, Vol.7 (7), p.917-923

1994

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Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8+ T-cell response

Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase

Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?

Recombinant single-chain Fv antibody fragment–alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization

Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses

Expression of recombinant alkaline phosphatase conjugates in Escherichia coli

Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection

Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody

Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin

BgK, a disulfide-containing sea anemone toxin blocking K + channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein

Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes

Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein 1 1Edited by R. Huber

Direct expression in E.coli of a functionally active protein A–snake toxin fusion protein

On the site by which α-dendrotoxin binds to voltage-dependent potassium channels: Site-directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

Three-dimensional crystal structure of recombinant erabutoxin a at 2.0 Å resolution

A Recombinant Insect‐Specific α‐Toxin of Buthus occitanus tunetanus Scorpion Confers Protection Against Homologous Mammal Toxins

A recombinant snake neurotoxin generated by chemical cleavage of a hybrid protein recovers full biological properties

Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence

A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short-chain variants

Mimicry between Receptors and Antibodies

Engineering of protein epitopes: a single deletion in a snake toxin generates full binding capacity to a previously unrecognized antibody

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