Details

Autor(en) / Beteiligte

• Biasucci, Luigi M
• Claudia, Mandolini
• Grimaldi, Maria Chiara
• La Rosa, Giulio
• Crea, Filippo

Titel

Abstract 18758: Epigenetic Regulation of Acute Coronary Syndromes

Ist Teil von

• Circulation (New York, N.Y.), 2016-11, Vol.134 (Suppl_1 Suppl 1), p.A18758-A18758

Ort / Verlag

by the American College of Cardiology Foundation and the American Heart Association, Inc

Erscheinungsjahr

2016

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• BackgroundLong noncoding RNAs (lncRNA) are long transcripts of more than 200 nt of length not encoding for proteins; they represent a novel source of molecules with a regulatory function at transcriptional and epigenetic level; only few information are available on their role in cardiology. As different mechanisms and prognosis have been described between type 2 Diabetic (T2DM) vs NotDiabetics patients with NSTEMI, with sought to assess whether LncRNA are involved in the different phenotypes in patients with and without diabetes.Material and MethodsPlatelet RNA from 3pts with NSTEMI without diabetes, 3 NSTEMI T2DM and 3 controls (CTRL ) were isolated for lncRNA and mRNA microarray study (ARRAYSTAR Inc USA).ResultsGlobal transcriptomic analyses detected 20.642 lncRNA and 18.949 mRNA in our three cohort of patients; we considered a p<0.001 and a fold induction >3. 5 as significant sign of up or down-regulation. In particular we found significant dysregulation ina)14 lncRNA in NSTEMI vs CTRL(9 up-regulated, 5 dysregulated), b)16 lncRNA, (6 up-regulated, 10 down-regulated) in NSTEMI T2DM vs CTRL and c) 356 lncRNA,(168 up-regulated, 188 down-regulated) in NSTEMI vs NSTEMI T2DM. Moreover,using the same definition of dysregulation), we found 8 mRNA,(4 up-regulated and 4 down-regulated), significantly dys-regulated in NSTEMI vs CTRL, 16 mRNA, (10 up-regulated, 6 down-regulated), in NSTEMI T2DM vs CTRL and 109 mRNA,(51 up-regulated, 58 down-regulated), in NSTEMI vs NSTEMI T2DM.. Dys-regulated transcripts were involved in calcium signalling pathway, fibrinolysis and platelet activation.ConclusionOur study suggests that LncRNA are pivotal player in NSTEMI because of the presence of an important dys-regulation at epigenetic level between patients with NSTEMI and CTRL; surprisingly, the larger differences were found between NSTEMI pts with and without diabetes, suggesting an important epigenetic affect in NSTEMI with T2DM .