Details

Autor(en) / Beteiligte

• Subramanian , Ganapathy
• Subramanian, Ganapathy

Titel

Very High Cell Density in Perfusion of CHO Cells by ATF, TFF, Wave Bioreactor, and/or CellTank Technologies – Impact of Cell Density and Applications

Ist Teil von

• Continuous Processing in Pharmaceutical Manufacturing, 2014, p.339-356

Ort / Verlag

Germany: Wiley

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Wiley Online Library All Obooks

Beschreibungen/Notizen

• This chapter presents a brief study on the following continuous disposable systems: (i) wave‐induced disposable bioreactor equipped with alternating tangential‐flow filtration (W‐ATF); (ii) wave‐induced disposable bioreactor equipped with tangential‐flow filtration (W‐TFF); and (iii) CellTank, nonwoven fiber matrix‐based bioreactor with integrated perfusion (CT). The goals of the study are to develop high‐cell density perfusion processes with these systems and then carry out performance evaluations of the systems and understand the limits of the technologies. Two Chinese hamster ovary (CHO) cell lines producing different monoclonal antibody were used: CHO#1 used with the W‐ATF and the W‐TFF, and CHO#2 used with the CT. The authors' strategy to develop the W‐ATF and the W‐TFF processes included the following steps: at first a learning phase including the development of the perfusion process and a study of the equipment, and then an evaluation of the limits of the system in terms of cell density.